Abstract:

To establish a direct enantioseparation method by HPLC on immobilized amylose-based chiral stationary phase for the chiral separation of the enantiomers of dracocephins A. The separation was carried out using a Chiralpak IA column (4.6mm×250mm, 5μm) in combination with n-hexane-ethyl acetate (10∶90) as mobile phase. The flow rate was 1.0mL·min-1 and detection wavelength was 290nm, whilst the column temperature was room temperature. The established chromatographic method was effective for the simultaneous separation of the two pairs of enantiomers of dracocephins A. The elution order was determined by coupling HPLC-CD detection and comparison with each single enantiomer. Immobilized amylosebased chiral stationary phase is suitable for the enantioseparation of dracocephins A.

Key words: chiral stationary phase, Chiralpak IA, dracocephins A, high-performance liquid chromatography