JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2016, Vol. 51 ›› Issue (3): 19-28.doi: 10.6040/j.issn.1671-9352.0.2015.458

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Research on genetic diversities of Asiatic beaver (Castor fiber birulai)on mtDNA D-loop HV-Ⅰin Xinjiang, China

DU Cong-cong1, 2, ZHAO Jing3, CHU Hong-jun1, 2, 4*, LIU Yuan-chao2,4,DUAN Xiao-nan1, 2, CHEN Gang2   

  1. 1.College of Life Science &
    Technology, Xinjiang University, Urumqi 830046, Xinjiang, China;
    2. Buergen Beaver National Nature Reserve, Altay 836500, Xinjiang, China;
    3. College of Biology Sciences, Shandong University, Jinan 250100, Shandong, China;
    4. College of Resources and Environment Sciences, Xinjiang University, Urumqi 830046, Xinjiang, China
  • Received:2015-09-23 Online:2016-03-20 Published:2016-04-07

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Abstract: Genetic diversities of Asiatic beaver(Castor fiber)in Xinjiang was investigated by analyzing the HV-Ⅰ sequences of the mitochondrial DNA control region(D-loop)of 16 individuals from the Ulungur watershed of Xinjiang in China. Results showed that the length of mtDNA D-loop HV-Ⅰ sequence of Xinjiang beaver was 569 bp, with nucleotide frequencies of 27.0%,32.7%,24.0% and 16.2% for A,T,G and C respectively, and the frequencies of A and T 山 东 大 学 学 报 (理 学 版)第51卷 - 第3期杜聪聪,等:新疆河狸mtDNA D-loop HV-Ⅰ区的遗传多样性研究 \=-was higher than that of G and C, which indicated the preference of nucleotides; that 10 polymorphic sites(2.03% of total analyzed sites)were found in the mtDNA D-loop HV-Ⅰ sequence of Xinjiang beaver compared with that(AY623632)of Mongolia beaver, of which there were four transitions sites, five transversions sites and one missing site; that Xinjiang beaver and Mongolia beaver were in one cluster by phylogenetic analyses based on the open mtDNA D-loop HV-Ⅰ sequence of beaver in other regions on GenBank, suggesting Xinjiang beaver also belonged to the subspecies of C. f. birulai; that Xinjiang beaver population had two unique haplotypes, whose haplotype diversity(Hd), nucleotide diversity(Pi)and average number of nucleotide differences(K)were 0.233 00±0.015 78, 0.001 23±0.015 90 and 0.700, indicating a low level of genetic diversity, deeply related to its limited environment and human factors.

Key words: Xinjiang beaver, genetic diversity, mtDNA D-loop

CLC Number: 

  • Q38
[1] 卢浩泉. 河狸(Castor fiber)在中国的分布与生态特点[J]. 山东大学学报(自然科学版),1981,16(4):103-109. LU Haoquan. Ecological characters og the chinese beaver[J]. Journal of Shandong University(Natural Sciences), 1981, 16(4):103-109.
[2] 陈道富,全仁哲,范喜顺,等. 欧亚河狸的生物学特性及其保护与开发[J]. 石河子大学学报(自然科学版),2003,7(1):84-86. CHEN Daofu, QUAN Renzhe, FAN Xishun, et al. Approach to the bionomics and value of protection and exploitation of Castor fiber[J]. Journal of Shihezi University(Natural Sciences Edition), 2003, 7(1):84-86.
[3] SEREBRENNIKOV M. Review of the beavers of the Palaearctic Region(Castor, Rodentia)[M]. Leningrad: Proceedings of the Academy of Sciences, 1929: 271-276.
[4] LAVROV L S. The beavers of the Palaeartic[M]. Voronezh: Izdatelstvo Voronezhskogo Universiteta, 1981(in Russian).
[5] DURKA W, BABIK W, DUCROZ J F, et al. Mitochondrial phylogeography of the Eurasian beaver Castor fiber L[J]. Molecular Ecology, 2005, 14(12):3843-3856.
[6] DJOSHKIN W W, SAFONOW W G. Die Biber der Alten und Neuen Welt[M]. Wittenberg: A Ziemsen Verlag, 1972: 1-168(in German).
[7] VERON G. Biogeographic history of the beaver(Castor-Fiber, Rodentia, Mammalia)[J]. Mammalia, 1992, 56(1):87-108.
[8] NOLET B A, ROSELL F. Comeback of the beaver Castor fiber: an overview of old and new conservation problems[J]. Biological Conservation, 1998, 83(2):165-173.
[9] LAVROV L S. Evolutionary developments of the genus Castor and taxonomy of the contemporary beavers of Eurasia[J]. Acta Zoologica Fennica, 1983, 174:87-90.
[10] HEIDECKE D. Taxonomical aspects of species conservation exemplified with the Eurasian beaver[J]. Hercynia N F 1986, 2, 146-161(in German).
[11] CHU H J, JIANG Z G. Distribution and conservation of the Sino-Mongolia beaver Castor fiber birulai in China[J]. Oryx, 2009, 43(2):197-202.
[12] 汪松.中国濒危动物红皮书(兽类)[M].北京:科学出版社, 1998. WANG Song. China red data book of endangered animals-Beasts[M]. Beijing: Science Press, 1998.
[13] 赵景辉,赵伟刚,陈玉山,等. 中国的河狸资源考察[J]. 特产研究,2005,29(3):38-41. ZHAO Jinghui, ZHAO Weigang, CHEN Yushan, et al. Observation and Study on Natural Resource in Chinese Beaver[J]. Special Wild Economic Animal and Plant Research, 2005, 29(3):38-41.
[14] 初红军. 新疆河狸考察[J]. 大自然,1993,14(4):12-13. CHU Hongjun. Mengxin Castor fiber investigation[J]. China Nature, 1993, 14(4):12-13.
[15] 刘冬至,蒋志刚,初红军,等. 蒙新河狸夏季夜间活动节律和时间分配[J]. 兽类学报,2013, 33(4):319-325. LIU Dongzhi, JIANG Zhigang, CHU Hongjun, et al. Summer nocturnal activity rhythms and time budgets of the Sino-Mongolia beaver(Castor fiber birulai) in Xinjiang, China[J]. Acta Theriologica Sinica, 2013, 33(4):319-325.
[16] 刘冬至,黄效文,初红军,等. 基于红外相机陷阱技术的蒙新河狸(Caster fiber birulai) 活动节律[J]. 干旱区研究,2015,32(1):205-211. LIU Dongzhi, HUANG Xiaowen, CHU Hongjun, et al. Activity rhythms of Sino-Mongolia beaver(Castor fiber birulai) measured with infrared camera traps in Xinjiang, China[J]. Arid Zone Research, 2015, 32(1):205-211.
[17] 于长青,卢浩泉,邵闻,等. 蒙新河狸的食性研究[J]. 林业科学,1994, 30(6):519-524. YU Changqing, LU Haoquan, SHAO Wen, et al. The feeding habit of beavers in Xinjiang[J]. Scientia Silvae Sinicae, 1994, 30(6):519-524.
[18] 葛炎,侯金雷. 河狸秋季采食贮存和冬季食性的初步观察[J]. 干旱区研究,2001,18(1):80. GE Yan, HOU Jinlei. Preliminary observation on storing food in autumn and feeding habit in winter of beavers[J]. Arid Zone Research, 2001,18(1):80.
[19] 盛和林,徐宏发,张恩迪. 新疆河狸及其河狸香[J]. 兽类学报,1990, 10(4):263-267. SHENG Helin, XU Hongfa, ZHANG Endi. Xinjiang beaver and castorium[J]. Acta Theriologica Sinica, 1990, 10(4):263-267.
[20] 初振辉,纪加义. 新疆河狸香的药用价值和性状鉴别[J]. 中草药,1992, 23(8):317-318. CHU Zhenhui, JI Jiayi. The medicinal value and character identification of Xinjiang Castor[J]. Chinese Traditional and Herbal Drugs, 1992, 23(8):317-318.
[21] 汪永庆. 河狸香的特点和利用研究[J]. 中草药,1993, 24(12):653-654. WANG Yongqing. The research of features and utilization of Castor[J]. Chinese Traditional and Herbal Drugs, 1993, 24(12):653-654.
[22] 陈季武,胡天喜,盛和林. 河狸香抗氧化作用[J]. 华东师范大学学报(自然科学版),1997(1):95-98. CHEN Jiwu, HU Tianxi, SHENG Helin. Effects of Castor antioxidation[J]. Journal of East China Noamal University(Natural Sciences Edition), 1997(1):95-98.
[23] 王永功,胡锡润,于沉鱼. 新疆省道320线改建对布尔根河河狸自然保护区生境的影响[J]. 新疆环境保护,2008, 30(2):20-23. WANG Yonggong, HU Xirun, YU Chenyu. Reconstructing Xinjiang provincial road 320 line influencing on habiat of beaver natural protective area in Buergen river Xinjiang[J]. Environmental Protection of Xinjiang, 2008, 30(2):20-23.
[24] 周继亮,张亚平,黄美华,等. 蝮亚科蛇线粒体细胞色素b基因序列分析及其系统发育[J]. 动物学报,2001, 47(4):361-366. ZHOU Jiliang, ZHANG Yaping, HUANG Meihua, et al. Phylogenetic relationships among crotalinae based on mitochondrial cytochrome b gene sequence variations[J]. Current Zoology, 2001, 47(4):361-366.
[25] 孙伟丽,杨博辉,曹学亮,等. 中国四个地方驴品种mtDNA D-Loop部分序列分析与系统进化研究[J]. 中国草食动物,2007, 27(2):7-10. SUN Weili, YANG Bohui,CAO Xueliang, et al. Phylogenetic relationship and genetic diversity of chinese four domestic donkeys using mtDNA D-Loop[J]. China Herbivores, 2007, 27(2):7-10.
[26] 冯慧,黄原,任轶,等. 陕西省林麝mtDNA D-loop区序列结构和种群遗传多样性[J]. 生态学报,2014, 34(20):5887-5895. FENG Hui, HUANG Yuan, REN Yi, et al. Structure of the mitochondrial DNA D-Loop region and genetic diversity of Moschus berezovskii in Shaanxi province[J]. Acta Ecological Sinica, 2014, 34(20):5887-5895.
[27] JEAN C T, HUI C F, LEE S C, et al. Variation in mitochondrial DNA and phylogenetic relationships of fishes of the subfamily Sparinae(Perciformes: Sparidae)in the coastal waters of Taiwan[J]. Zoological Studies,1995, 34(4):270-280.
[28] SACCONE C, ATTIMONELLI M, SBISA E. Structural elements highly preserved during the evolution of the D-loop-containing region in vertebrate mitochondrial-DNA[J]. Journal of Molecular Evolution, 1987, 26(3):205-211.
[29] 齐国强. 中国部分地方水牛品种mtDNA D-loop区遗传多样性与起源研究[D]. 陕西:西北农林科技大学,2007. QI Guoqiang. Mitochondrial DNA D-loop genetic diversity and origin of some chinese domestic buffalo breeds[D]. Shaanxi: Northwest A&F University, 2007.
[30] LOFTUS R T, MACHUGH D E, BRADLEY D G, et al. Evidence for two independent domestications of cattle[J]. Proc Natl Acds Sci USA, 1994, 91(7):2757-2761.
[31] 龚金波,苏天凤,夏军红,等. 中国近海黑鲷线粒体DNA控制区序列多态性分析[J]. 南方水产,2006, 2(4):24-30. GONG Jinbo, SU Tianfeng, XIA Junhong, et al. Polymorphism study of the mitochondrial DNA D-loop control region sequences from black porgy Acanthopagrus schlegeli in the costal waters of China[J]. South China Fisheries Science, 2006, 2(4):24-30.
[32] 张洪海,窦海龙,陈磊,等.中国狼不同地理种群遗传多样性及系统进化分析[J]. 生态学报,2015,35(6):1869-1881. ZHANG Honghai, DOU Hailong, CHEN Lei, et al. Genetic diversity and phylogenetic analysis of wolves from different geographical regions of China[J]. Acta Ecologica Sinica, 2015, 35(6):1869-1881.
[33] LUNT D H, WHIPPLE L E, HYMAN B C. Mitochondrial DNA variable number tandem repeats(VNTRs): utility and problems in molecular ecology[J]. Molecular Ecology, 1998, 7(11):1441-1455.
[34] CHENEY L C. An assessment of genetic variation within and between sea otter(Enhydra lutris)populations off Alaska and California[D]. San Jose: Moss Landing Marine Laboratories, California State University, 1995.
[35] VILÀ C, AMORIM I R, LEONARD J A, et al. Mitochondrial DNA phylogeography and population history of the grey wolf Canis lupus[J]. Molecular Ecology, 1999, 8(12):2089-2103.
[36] DUCROZ J F, STUBBE M, SAVELJEV A P, et al. Genetic variation and population structure of the eurasian beaver Castor fiber in eastern europe and asia[J]. Journal of Mammalogy, 2005, 86(6):1059-1067.
[37] 李娜,陈少波,谢起浪,等. 闽浙地区香鱼线粒体Cytb基因和D-loop区序列多态性分析[J]. 遗传,2008, 30(7):919-925. LI Na, CHEN Shaobo, XIE Qilang, et al. Polymorphisms of mitochondrial Cyt b gene and D-loop region in sweetfish(Plecoglossus altivelis Temminck et Schlegel) from Zhejiang and Fujian provinces[J]. Hereditas(Beijing), 2008, 30(7):919-925.
[38] 张成福,徐利娟,姬秋梅,等. 西藏牦牛mtDNA D-loop区的遗传多样性及其遗传分化[J]. 生态学报,2012, 32(5):1387-1395. ZHANG Chengfu, XU Lijuan, JI Qiumei, et al. Genetic diversity and evolution relationship on mtDNA D-loop in Tibet anyaks[J]. Acta Ecologica Sinica, 2012, 32(5):1387-1395
[39] 刘元超,赵晶,初红军,等. 蒙古国西北部狼(Canis lupus)遗传多样性研究[J]. 山东大学学报(理学版),2015, 50(1):42-49. LIU Yuanchao, ZHAO Jing, CHU Hongjun, et al. Genetic diversities of wolves mtDNA D-Loop in northwestern Mongolia[J]. Journal of Shandong University(Natural Science), 2015, 50(1):42-49.
[40] BANDELT H J, FORSTER P, RÖHL A. Median-joining networks for inferring intraspecific phylogenies[J]. Molecular biology and evolution, 1999, 16(1):37-48.
[41] ROZAS J, SÁNCHEZ-DELBARRIO J C, MESSEGUER X, et al. DnaSP, DNA polymorphism analyses by the coalescent and other methods[J]. Bioinformatics, 2003, 19(18):2496-2497.
[42] NEI J E, AVISE J C. Application of a random walkmodel to geographic distributions of animal mitochondrial DNA variation[J]. Genetics, 1993, 135(4):1209-1220.
[43] 彭珊,代应贵. 濒危鱼类稀有白甲鱼清水江种群mtDNA D-loop序列多态性[J]. 水产学报,2009, 33(2):196-200. PENG Shan, DAI Yinggui. Sequence polymorphism of mtdna d-loop in the population of the endangered species onychostoma rara from the Qingshui river[J]. Journal of Fisheries of China, 2009, 33(2):196-200.
[44] 张丽,滚双宝,雷天云,等. 应用mtDNA Cytb基因全序列分析中国5个马鹿群体的遗传多样性和系统发育[J]. 华北农学报,2010, 25(4):12-16. ZHANG Li, GUN Shuangbao, LEI Tianyun, et al. Analysis of genetic diversity and phylogeny of five chinese subspecies of wapiti using mitochondrial cyt b complete sequence[J]. Acta Agriculturae Boreali-Sinica, 2010, 25(4):12-16.
[45] LAN H, SHI L. The origin and genetic differentiation of native breeds of pigs in southwest China: an approach from mitochondrial DNA polymorphism[J]. Biochemical genetics, 1993, 31(1/2):51-60.
[46] 门凤歧,赵祥麟. 古生物学导论[M]. 北京:地质出版社,1993. MENG Fengqi, ZHAO Xianglin. Introduction to Ancient Biology[M]. Beijing: Geological Publishing House, 1998.
[47] PARKER P G, WHITEMAN H H. Genetic diversity in fragmented populations of clemmys guttata and chrysemys picta marginata as shown by DNA fingerprinting[J]. Copeia, 1993(3):841-846.
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