%A YU Hui-min1, SHI Zhu1, SHAO Hong-wei1, LI Wei1, HOU Bing-kai2* %T  Agrobacterium-mediated introduction of disease-resistant genes
into tomato(Lycopersicon esculentum Mill %0 Journal Article %D 2011 %J JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) %R %P 1-4 %V 46 %N 11 %U {http://lxbwk.njournal.sdu.edu.cn/CN/abstract/article_2196.shtml} %8 2011-11-20 %X

A plant expression vector pBLGC containing two disease-resistant genes, the Chitinase gene and the Glutanase gene, was introduced into cotyledon explants of tomato via Agrobacterium-mediated genetic transformation. After co-culture, kanamycin selection and regeneration, 23 kanamycin-resistant plants were obtained. Among these, 7 plants were demonstrated to be transgenics by PCR and PCR-Southern Blot analysis. Through pathogenic fungus inoculation, it was shown that 3 transgenic plants had strong disease resistance. These results will lay an important basis for the further study of tomato disease resistance and for tomato genetic improvement.