J4 ›› 2012, Vol. 47 ›› Issue (3): 10-13.
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XU Li-hong, LUO Shen-heng, WANG Sha-sha, HOU Guo-li, DIAO Ai-po*
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Abstract:
To study expression and function of PMEPA1 protein in cells, the constructs of pTrcHisA-PMEPA1 and pGEX4T-2-PMEPA1 were made and transformed into E.coli BL21RIPL to express recombinant protein His-PMEPA1 and GSTpPMEPA1. The proteins were purified by Ni-TNA and Glutathione Sepharose 4B affinity chromatography respectively. A rabbit was immunized with purified His-PMEPA1, the antibody against PMEPA1 was prepared successfully, and purified using GST-PMEPA1. Western blotting showed that the purified antibody can recognize endogenous PMEPA1 proteins in cell lines.
Key words: GST-PMEPA1; His-PMEPA1; prokaryotic expression;antibody; purification
XU Li-hong, LUO Shen-heng, WANG Sha-sha, HOU Guo-li, DIAO Ai-po*. Expression and purification of PMEPA1 protein and its antibody preparation[J].J4, 2012, 47(3): 10-13.
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