JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2016, Vol. 51 ›› Issue (3): 19-28.doi: 10.6040/j.issn.1671-9352.0.2015.458

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Research on genetic diversities of Asiatic beaver (Castor fiber birulai)on mtDNA D-loop HV-Ⅰin Xinjiang, China

DU Cong-cong1, 2, ZHAO Jing3, CHU Hong-jun1, 2, 4*, LIU Yuan-chao2,4,DUAN Xiao-nan1, 2, CHEN Gang2   

  1. 1.College of Life Science &
    Technology, Xinjiang University, Urumqi 830046, Xinjiang, China;
    2. Buergen Beaver National Nature Reserve, Altay 836500, Xinjiang, China;
    3. College of Biology Sciences, Shandong University, Jinan 250100, Shandong, China;
    4. College of Resources and Environment Sciences, Xinjiang University, Urumqi 830046, Xinjiang, China
  • Received:2015-09-23 Online:2016-03-20 Published:2016-04-07

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Abstract: Genetic diversities of Asiatic beaver(Castor fiber)in Xinjiang was investigated by analyzing the HV-Ⅰ sequences of the mitochondrial DNA control region(D-loop)of 16 individuals from the Ulungur watershed of Xinjiang in China. Results showed that the length of mtDNA D-loop HV-Ⅰ sequence of Xinjiang beaver was 569 bp, with nucleotide frequencies of 27.0%,32.7%,24.0% and 16.2% for A,T,G and C respectively, and the frequencies of A and T 山 东 大 学 学 报 (理 学 版)第51卷 - 第3期杜聪聪,等:新疆河狸mtDNA D-loop HV-Ⅰ区的遗传多样性研究 \=-was higher than that of G and C, which indicated the preference of nucleotides; that 10 polymorphic sites(2.03% of total analyzed sites)were found in the mtDNA D-loop HV-Ⅰ sequence of Xinjiang beaver compared with that(AY623632)of Mongolia beaver, of which there were four transitions sites, five transversions sites and one missing site; that Xinjiang beaver and Mongolia beaver were in one cluster by phylogenetic analyses based on the open mtDNA D-loop HV-Ⅰ sequence of beaver in other regions on GenBank, suggesting Xinjiang beaver also belonged to the subspecies of C. f. birulai; that Xinjiang beaver population had two unique haplotypes, whose haplotype diversity(Hd), nucleotide diversity(Pi)and average number of nucleotide differences(K)were 0.233 00±0.015 78, 0.001 23±0.015 90 and 0.700, indicating a low level of genetic diversity, deeply related to its limited environment and human factors.

Key words: Xinjiang beaver, genetic diversity, mtDNA D-loop

CLC Number: 

  • Q38
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