不同碳源下Pseudomonas putida F1单环芳烃降解相关蛋白质的差异表达

J4 ›› 2013, Vol. 48 ›› Issue (05): 14-19.

不同碳源下Pseudomonas putida F1单环芳烃降解相关蛋白质的差异表达

张晓,张玉臻,刘军军,刘红蕾*

山东大学生命科学学院, 山东济南 250100

收稿日期:2012-11-10 出版日期:2013-05-20 发布日期:2013-05-10
通讯作者: 刘红蕾(1971- ),女,讲师,研究方向为资源与环境微生物.Email: lhl@sdu.edu.cn
作者简介:张晓(1986- ),女,博士研究生,研究方向为资源与环境微生物.Email:zhangxiao3221863@163.com
基金资助:
山东省优秀中青年科学家科研奖励基金资助项目 (2007BS08019)

Differential expression of aromatic hydrocarbon degradation related proteins in Pseudomonas putida F1 grown on succinate, toluene or ethylbenzene

ZHANG Xiao, ZHANG Yu-zhen, LIU Jun-jun, LIU Hong-lei*

School of Life Sciences, Shandong University, Jinan 250100, Shandong, China

Received:2012-11-10 Online:2013-05-20 Published:2013-05-10

摘要/Abstract

摘要：

研究了Pseudomonas putida F1利用单环芳香族化合物以及琥珀酸做碳源时单环芳烃降解相关蛋白质的表达,这对于研究这些芳烃的单一和混合降解有重要意义。利用蛋白组学研究方法,通过比较蛋白表达的双向凝胶电泳(2-DGE)图谱,找出不同碳源条件下生长细胞中差异表达的蛋白质。蛋白质经胰酶消化后,进行基质辅助激光解吸电离飞行时间质谱 (Matrix assisted laser desorption／ionization-Time of flight (MALDI-TOF) mass spectrometry)分析。结果表明,尽管该细菌在降解甲苯和乙苯时利用同一个代谢途径,但代谢相关蛋白在微生物利用不同碳源时的表达量有所变化。差异表达蛋白可分为4类,代谢蛋白,转运蛋白,适应性相关蛋白以及其他类型蛋白。

关键词: 蛋白组学;双向凝胶电泳;MALDI-TOF;单环芳烃; 蛋白质表达

Abstract:

The degradation of aromatic compounds has been widely studied biochemically or genetically. Through 2-Dimensional gel electrophoresis (2-DGE) and Matrix assisted laser desorption／ionization-Time of flight (MALDI-TOF) mass spectrometry analysis, the expression of mono cyclic aromatic compounds degradation related proteins were compared when bacteria Pseudomonas putida F1 was grown on succinate, touene, ethylbenzene or the mixture of toluene and ethylbenzene as carbon and energy sources. Although the aromatic compounds were all metabolized using TOD pathway, the expression of some aromatic hydrocarbon degradation related proteins were different when the cells were grown on different carbon sources. Four groups of differentially expressed proteins have been identified, including metabolic proteins, transportation proteins, adaptation related proteins and other proteins.

Key words: proteomics; 2-DGE; MALDI-TOF; monocyclic aromatic hydrocarbon; protein expression

张晓,张玉臻,刘军军,刘红蕾*. 不同碳源下Pseudomonas putida F1单环芳烃降解相关蛋白质的差异表达[J]. J4, 2013, 48(05): 14-19.

ZHANG Xiao, ZHANG Yu-zhen, LIU Jun-jun, LIU Hong-lei*. Differential expression of aromatic hydrocarbon degradation related proteins in Pseudomonas putida F1 grown on succinate, toluene or ethylbenzene[J]. J4, 2013, 48(05): 14-19.

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