冷冻干燥过程中体系pH模拟与可视化测量

J4 ›› 2013, Vol. 48 ›› Issue (05): 39-42.

冷冻干燥过程中体系pH模拟与可视化测量

李春龙1,2,李小爽1,徐霞1*

1.中国科学院过程工程研究所生化工程国家重点实验室, 北京 100190;
2.中国科学院大学, 北京 100049

收稿日期:2013-03-01 出版日期:2013-05-20 发布日期:2013-05-10
通讯作者: 徐霞（1969- ），女，研究员，博士生导师，主要从事生化剂型化及体外治疗技术研究. Email：xuxia@home.ipe.ac.cn
作者简介:李春龙（1988- ），男，硕士研究生，主要从事生物大分子冷冻干燥研究. Email：chlli@home.ipe.ac.cn

Mathematical and visual approaches for determining pH during freeze-drying

LI Chun-long1,2, LI Xiao-shuang1, XU Xia1*

1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences,
Beijing 100190, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2013-03-01 Online:2013-05-20 Published:2013-05-10

摘要/Abstract

摘要：

在冷冻干燥过程中,体系pH是维持生物大分子稳定性的重要因素。本研究基于Mazur方程建立溶液降温和结晶过程的pH变化方程,计算得到不同缓冲体系在冷冻过程中的pH变化曲线;利用荧光显微镜观测在荧光素钠存在下冷冻过程中体系的pH变化,并通过图像分析获取降温过程中体系的pH值,结果均与文献测量值吻合。研究结果表明冷冻过程中体系的pH变化可通过体系的物化性质进行预测,并可通过非侵入型的可视法进行实时测量。

关键词: 冷冻干燥;非侵入型方法;pH变化方程

Abstract:

pH changes during freezing played a critical role in maintaining the biostabilization of biomacromolecules after lyophilization. In this study a mathematics model based on the Mazur equation was applied to explore pH changes during freezing. The pH change with temperature and time during freezing was simulated for different buffer systems. Fluorescence images under different conditions were taken using a fluorescence microscopy during freezing. pH was determined using an image analysis method. The simulation results revealed that the pH value of the system during freezing can be predicted using the mathematic model. The non-invasive method developed is able to measure the system pH during freezing.

Key words: freeze-drying; non-invasive approach; pH change equation

李春龙1,2,李小爽1,徐霞1*. 冷冻干燥过程中体系pH模拟与可视化测量[J]. J4, 2013, 48(05): 39-42.

LI Chun-long1,2, LI Xiao-shuang1, XU Xia1*. Mathematical and visual approaches for determining pH during freeze-drying[J]. J4, 2013, 48(05): 39-42.

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