关键词: 选择性标记去除, FLP/frt, 单子叶植物 , Ubi, Actin1, 位点特异性重组系统

Abstract: Abstract: The FLP/frt sitespecific recombination system of the 2?μm plasmid of Saccharomyces cerevisiae can be applied to eliminate the marker gene from the nuclear genome after successful selection of the transgenic plants (cells). The promoters of the rice actin gene and maize ubiquitin gene can efficiently regulate the constitutive expression of a foreign gene in transgenic monocotyledons. The FLP/frt sitespecific recombination system fitting for monocotyledonous crops was constructed to breed the markerfree transgenic monocotyledons with high tolerance to salt and drought. This system includes the FLP expression vector pCAMBIA3300UbiFLPbar, the frt containing vectors pCAMBIA1300UbiTsPPasefrtalsfrtm and pCAMBIA 1300Actin1AtNHX1frtalsfrtm.

Key words: Monocotyledonous plants , Ubi, Actin1, sitespecific recombination system, FLP/frt, delection of selectable marker