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山东大学学报（理学版） ›› 2016, Vol. 51 ›› Issue (3): 19-28.doi: 10.6040/j.issn.1671-9352.0.2015.458

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新疆河狸mtDNA D-loop HV-Ⅰ区的遗传多样性研究

杜聪聪1, 2,赵晶3,初红军1, 2, 4*,刘元超2, 4,端肖楠1, 2,陈刚2   

  1. 1.新疆大学生命科学与技术学院, 新疆 乌鲁木齐 830046;2.新疆布尔根河狸国家级自然保护区管理站, 新疆 阿勒泰 836500;3.山东大学生命学院, 山东 济南 250100;4.新疆大学资源与环境科学学院, 新疆 乌鲁木齐 830046
  • 收稿日期:2015-09-23 出版日期:2016-03-20 发布日期:2016-04-07
  • 通讯作者: 初红军(1964— ),男,高级工程师,博士生导师,研究方向为动物生态和濒危物种保护生物学. E-mail:hongjunchu@vip.163.com E-mail:Cleverdu@126.com
  • 作者简介:杜聪聪(1990— ),男,硕士研究生,研究方向为动物生态和濒危物种保护生物学. E-mail:Cleverdu@126.com
  • 基金资助:
    新疆维吾尔自治区国际科技合作项资助项目(20136026-2);科技部科技基础性工作专项重大资助项目(2013FY110300);新疆布尔根河狸国家级自然保护区2014年能力建设补助项目

Research on genetic diversities of Asiatic beaver (Castor fiber birulai)on mtDNA D-loop HV-Ⅰin Xinjiang, China

DU Cong-cong1, 2, ZHAO Jing3, CHU Hong-jun1, 2, 4*, LIU Yuan-chao2,4,DUAN Xiao-nan1, 2, CHEN Gang2   

  1. 1.College of Life Science &
    Technology, Xinjiang University, Urumqi 830046, Xinjiang, China;
    2. Buergen Beaver National Nature Reserve, Altay 836500, Xinjiang, China;
    3. College of Biology Sciences, Shandong University, Jinan 250100, Shandong, China;
    4. College of Resources and Environment Sciences, Xinjiang University, Urumqi 830046, Xinjiang, China
  • Received:2015-09-23 Online:2016-03-20 Published:2016-04-07

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摘要： 通过测定和分析我国新疆乌伦古河流域16只河狸的mtDNA D-loop HV-Ⅰ区序列,对新疆河狸的遗传多样性进行了研究。结果表明,新疆河狸mtDNA D-loop HV-Ⅰ区序列长度为569 bp, A、T、G和C四种核苷酸的平均比例分别为27.0%、32.7%、24.0%和16.2%,新疆河狸mtDNA D-loop HV-Ⅰ区A+T含量高于G+C含量,表现出碱基组成的偏倚性。与蒙古国河狸mtDNA D-loop HV-Ⅰ区(AY623632)比对后,发现我国新疆河狸mtDNA D-loop HV-Ⅰ区10个变异位点,占分析位点总数的2.03%,其中转换位点4个,颠换位点5个,缺失位点1个,无插入位点。依据GenBank上已公布的不同地区河狸种群mtDNA D-loop HV-Ⅰ区序列,构建系统树后发现我国新疆河狸和蒙古国河狸位于一个分支,说明两者属于欧亚河狸种下的同一个亚种-蒙新亚种(Castor fiber birulai)。在16只新疆河狸个体中鉴定出2种单倍型,单倍型多样性(Hd)为0.233 00±0.015 78,核苷酸多样性(Pi)为0.001 23±0.015 90,平均核苷酸差异数(K)为0.700,与其他河狸亚种种群比较后发现新疆河狸遗传多样性较低,基因丰富度低,这与新疆河狸局限的生活环境和人为因素的影响是密切相关的。

关键词: mtDNA D-loop HV-Ⅰ区, 遗传多样性, 新疆河狸

Abstract: Genetic diversities of Asiatic beaver(Castor fiber)in Xinjiang was investigated by analyzing the HV-Ⅰ sequences of the mitochondrial DNA control region(D-loop)of 16 individuals from the Ulungur watershed of Xinjiang in China. Results showed that the length of mtDNA D-loop HV-Ⅰ sequence of Xinjiang beaver was 569 bp, with nucleotide frequencies of 27.0%,32.7%,24.0% and 16.2% for A,T,G and C respectively, and the frequencies of A and T 山 东 大 学 学 报 (理 学 版)第51卷 - 第3期杜聪聪,等:新疆河狸mtDNA D-loop HV-Ⅰ区的遗传多样性研究 \=-was higher than that of G and C, which indicated the preference of nucleotides; that 10 polymorphic sites(2.03% of total analyzed sites)were found in the mtDNA D-loop HV-Ⅰ sequence of Xinjiang beaver compared with that(AY623632)of Mongolia beaver, of which there were four transitions sites, five transversions sites and one missing site; that Xinjiang beaver and Mongolia beaver were in one cluster by phylogenetic analyses based on the open mtDNA D-loop HV-Ⅰ sequence of beaver in other regions on GenBank, suggesting Xinjiang beaver also belonged to the subspecies of C. f. birulai; that Xinjiang beaver population had two unique haplotypes, whose haplotype diversity(Hd), nucleotide diversity(Pi)and average number of nucleotide differences(K)were 0.233 00±0.015 78, 0.001 23±0.015 90 and 0.700, indicating a low level of genetic diversity, deeply related to its limited environment and human factors.

Key words: Xinjiang beaver, genetic diversity, mtDNA D-loop

中图分类号: 

  • Q38
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[1] 刘元超, 赵晶, 初红军, 董潭成, 韩丽丽, 陶永善, 葛炎. 蒙古国西北部狼(Canis lupus)遗传多样性研究[J]. 山东大学学报（理学版）, 2015, 50(01): 42-49.
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