水溶性海星皂苷的分离纯化及其抗肿瘤活性研究

J4 ›› 2013, Vol. 48 ›› Issue (1): 30-35.

水溶性海星皂苷的分离纯化及其抗肿瘤活性研究

赵君1,赵晶2,樊廷俊1*,袁文鹏1,3,张铮1,丛日山1

1. 中国海洋大学海洋生命学院海洋生物系, 山东青岛 266003;
2. 山东大学生命科学学院生物系, 山东济南 250100; 3. 山东省科学院生物研究所,山东济南 250014

收稿日期:2012-05-18 出版日期:2013-01-20 发布日期:2013-01-15
通讯作者: 樊廷俊(1964- ), 男, 博士, 教授, 博士生导师, 主要研究方向为细胞工程. Email: tjfan@ouc.edu.cn
作者简介:赵君(1983- ), 女, 博士, 讲师, 主要研究方向为细胞工程. Email: zhaojun@ouc.edu.cn
基金资助:
青岛市科技发展计划项目自然基金资助项目(04-2-JZ-82)

Purification and anti-tumor activity examination of water-soluble asterosaponin from Asterias rollestoni Bell

ZHAO Jun1, ZHAO Jing2, FAN Ting-jun1*, YUAN Wen-peng1,3, ZHANG Zheng1, CONG Ri-shan1

1. Department of Marine Biology, College of Marine Life Science, Ocean University of China, Qingdao 266003,
Shandong, China; 2. College of Life Science, Shandong University, Jinan 250100, Shandong, China;
3. Research Institute of Biology, Science Academy of Shandong, Jinan 250014, Shandong, China

Received:2012-05-18 Online:2013-01-20 Published:2013-01-15

摘要/Abstract

摘要：

为利用废弃海星壳资源开发出纯天然抗肿瘤药物,通过AB-8型吸附树脂、常压硅胶与Sephadex LH-20葡聚糖凝胶的柱层析技术,从罗氏海盘车(Asterias rollestoni Bell)壳的水抽提液中分离纯化出水溶性海星皂苷,并对其体内外抑瘤活性进行了研究。分离纯化与性质鉴定的结果显示,所得水溶性海星皂苷的性质隶属于甾体皂苷,其纯化样品的纯度可高达99.6％;体外抑瘤活性检测结果证实,所得皂苷纯品对HeLa宫颈癌细胞、SGC-7901胃癌细胞、A-549肺癌细胞和Bel7402肝癌细胞均具有显著的抑制活性。50mg／L皂苷对这4种癌细胞的抑制率分别为(92.06±2.60)％、(95.22±2.87)％、(95.04±3.30)％和(91。60±4。30)％,其IC50值分别为7.05、5.28、5.32、6.16mg／L。体内抑瘤活性检测结果证实,灌胃剂量为2、 5、 10μg／g的皂苷纯品对S180实体瘤荷瘤小鼠均具有显著的抑制活性,并具有浓度依赖性,10μg／g皂苷纯品的抑瘤率可高达46.27％,高于10μg／g抗肿瘤药物环磷酰胺(CTX)的抑瘤率(41.04％);同时,2、 5、 10μg／g的皂苷纯品还均能浓度依赖性地显著提高荷瘤小鼠的胸腺指数和脾指数。

关键词: 罗氏海盘车;水溶性海星皂苷;抑瘤活性;胸腺指数;脾指数

Abstract:

To develop natural antitumor medicaments from abandoned starfish shells, watersoluble asterosaponin from Asterias rollestoni Bell were isolated, purified and their antitumor bioactivities were examined. After extracted with hot water and purified with macroporous resin column and silica gel column chromatograghy respectively, watersoluble asterosaponin with purity of 99.6％ and properties of steroidal saponins was obtained. In vitro antitumor activity examination showed that the purified asterosaponin had high antitumor activity on HeLa cells, SGC7901 gastric cancer cells, A549 lung cancer cells and Bel7402 hepatocarcinoma cells. The inhibiting rate of 50 mg／L asterosaponin to the above four kinds of cancer cells was(92.06±2.60)％, (95.22±2.87)％, (95.04±3.30)％ and (91.60±4.30)％, and its corresponding IC50 value to these cells was 7.05, 5.28, 5.32 and 6.16mg／L, respectively. In vivo antitumor activity examination showed that the purified asterosaponin at the dose of 2.10μg／g bodyweight had obvious inhibiting effect on the S180 solid tumor in mice in a dose dependent manner. And tumorinhibiting rate of 10μg／g asterosaponin was as high as 46.27％, higher than that of 10μg／g cyclophosvnamide (CTX, one kind of anticancer drugs). Besides, 2.10μg／g asterosaponin could obviously increase the thymus index and spleen index of the tumor-bearing mice.

Key words: Asterias rollestoni Bell; water-soluble asterosaponin; antitumor activities; thymus index; spleen index

赵君1,赵晶2,樊廷俊1*,袁文鹏1,3,张铮1,丛日山1. 水溶性海星皂苷的分离纯化及其抗肿瘤活性研究[J]. J4, 2013, 48(1): 30-35.

ZHAO Jun1, ZHAO Jing2, FAN Ting-jun1*, YUAN Wen-peng1,3, ZHANG Zheng1, CONG Ri-shan1. Purification and anti-tumor activity examination of water-soluble asterosaponin from Asterias rollestoni Bell[J]. J4, 2013, 48(1): 30-35.

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