石菖蒲挥发油鼻用温敏型原位凝胶的制备

doi:10.6040/j.issn.1671-9352.0.2025.115

摘要/Abstract

摘要： 本研究探究制备石菖蒲挥发油鼻用温敏型原位凝胶工艺。采用正交设计法优化石菖蒲挥发油包合工艺参数,且通过饱和水溶液法对石菖蒲挥发油进行包合处理,并以泊洛沙姆407(P407)和泊洛沙姆188(P188)的质量分数为关键考察因素,以相变温度为指标,采用星点设计-效应面法进行二项式拟合。在此基础上,进一步优化石菖蒲挥发油鼻用温敏型原位凝胶的制备工艺,并对其抑制γ-氨基丁酸转氨酶(γ-aminobutyric acid transaminase, GABA-T)的活性进行评估。结果表明,石菖蒲挥发油的最佳包合工艺参数为挥发油与β-环糊精(β-CD)的投料比1 mL∶5 g,β-CD与H₂O的投料比1 g∶35 mL,包合温度30 ℃。当P407的质量分数为19.13%、P188的质量分数为1.08%时,所制备的原位凝胶相变温度为32 ℃,具有良好的应用前景。此外,该凝胶对GABA-T表现出剂量依赖性的抑制作用,其半数抑制浓度(IC₅₀)为215.7 μg/mL。本研究建立的石菖蒲挥发油温敏型鼻用原位凝胶制备工艺高效且可行,为石菖蒲挥发油的开发及其在抗神经系统疾病中的应用提供坚实的理论依据和技术支持。

关键词: 石菖蒲, 星点设计-效应面法, 原位凝胶, 挥发油

Abstract: This study successfully prepared a nasal thermosensitive in situ gel of Acorus tatarinowii volatile oil. The complexation process parameters of Acorus tatarinowii volatile oil were optimized using orthogonal design method. Meanwhile, taking the mass fractions of poloxamer 407(P407)and poloxamer 188(P188)as the key factors and phase transition temperature as the index, the binomial fitting was carried out using central composite design-response surface methodology. Based on this, the preparation process of the nasal thermosensitive in situ gel was further optimized, and its inhibitory activity against γ-aminobutyric acid transaminase(GABA-T)was assessed. The results indicated that the optimal inclusion complexation process parameters for Acorus tatarinowii volatile oil were as follows: the feeding ratio of volatile oil to β-cyclodextrin(β-CD)was 1 mL∶5 g, the feeding ratio of β-CD to H₂O was 1 g∶35 mL, and the complexation temperature was 30 ℃. When the mass fraction of P407 was 19.13% and the mass fraction of P188 was 1.08%, the phase transition temperature of the prepared in situ gel was 32 ℃, which had a promising application prospect. Moreover, the gel exhibited a dose-dependent inhibitory effect on GABA-T, with an IC₅₀ value of 215.7 μg/mL. The thermosensitive nasal in situ gel preparation process established in this study was efficient and feasible, providing a solid theoretical basis and technical support for the development of Acorus tatarinowii volatile oil and its application in the treatment of nervous system diseases.

Key words: Acorus tatarinowii Schott, central composite design-response surface methodology, in situ gel, volatile oil

中图分类号:

R285

陈亮,杜立霖,肖雅心,李嘉欣. 石菖蒲挥发油鼻用温敏型原位凝胶的制备[J]. 《山东大学学报(理学版)》, 2025, 60(9): 24-30.

CHEN Liang, DU Lilin, XIAO Yaxin, LI Jiaxin. Preparation of Acorus tatarinowii volatile oil nasal thermosensitive in situ gel[J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2025, 60(9): 24-30.

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