水溶性海星皂苷的分离纯化及其抗真菌活性研究

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水溶性海星皂苷的分离纯化及其抗真菌活性研究

樊廷俊¹,张铮¹,袁文鹏^1,2,丛日山¹,王卫卫¹,荆昭¹

1. 中国海洋大学海洋生命学院海洋生物系, 山东青岛 266003;
2. 山东省科学院生物研究所, 山东济南 250014

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-10-24 发布日期:2006-10-24
通讯作者: 樊廷俊

Purification and antifungal activity examination of water-soluble starfish glycosides from Asterias rollestoni

FAN Ting-jun¹, ZHANG Zheng¹, YUAN Wen-peng ^1,2, CONG Ri-shan¹, WANG Wei-wei¹, JING Zhao¹

1. Department of Marine Biology, College of Marine Life Science, Ocean University of China, Qingdao 266003, Shandong, China;
2. Research Institute of Biology, Science Academy of Shandong, Jinan 250014, Shandong, China

Received:1900-01-01 Revised:1900-01-01 Online:2006-10-24 Published:2006-10-24
Contact: FAN Ting-jun

摘要/Abstract

摘要： 为了分离纯化出具有强抗真菌活性的水溶性海星皂苷,以罗氏海盘车(Asterias rollestoni)腕为实验材料,利用水抽提方法获得水溶性海星皂苷粗品,依次利用大孔树脂和硅胶柱层析对水溶性海星皂苷进行了纯化,并对其抗真菌活性进行了测定。研究结果发现,在30％、50％、70％、80％、95％乙醇溶液的大孔树脂柱层析洗脱组分中,70％乙醇溶液洗脱组分对白色念珠菌和裂殖酵母菌的抗真菌活性最强。70％乙醇溶液洗脱组分经硅胶柱层析进一步纯化后,得到了SF-1、SF-2、SF-3和SF-4四种纯化样品,其中SF-1纯化样品没有抗真菌活性,SF-2纯化样品具有极弱的抗真菌活性,SF-3和SF-4纯化样品均具有很强的抗真菌活性。SF-3纯化样品在高压液相柱层析(HPLC)图谱中仅显示单一洗脱峰,表明其纯度很高,可应用于高效抗真菌药物的研制。研究结果为利用水溶性海星皂苷研制高效抗真菌药物奠定了基础。

关键词: 水溶性海星皂苷, 硅胶柱薄层层析, 大孔树脂柱层析, 抗真菌活性

Abstract: To exploit highly bioactive natural antifungal medicaments from starfish, water-soluble starfish glycosides from Asterias rollestoni were isolated and purified by macroporous resin column and silica gel column chromatography, and their antifungal bioactivities were also examined. Among the macroporous resin fractions of 30％, 50％, 70％, 80％ and 95％ ethanol, 70％ ethanol eluted fractions showed the highest antifungal activity towards Schizosaccharomyces pombe and Candida albicans. After being checked by thin layer chromatography, purified SF-1, SF-2, SF-3 and SF-4 fractions were obtained from 70％ ethanol eluted fractions with a silica gel column. Among the purified fractions, SF-3 and SF-4 showed much higher antifungal activities, while SF-1 did not show any antifungal activities and SF-2 had only very lower antifungal activities. SF-3 exhibited a single peak after loaded onto a high pressure liquid chromatography (HPLC) column, which indicated that SF-3 was pure enough to be used as a candidate of antifungal medicament development. This research will lay a solid foundation for the development and application of natural and highly bioactive antifungal medicaments.

Key words: silica gel column, macroporous resin column, antifungal activities, water-soluble starfish glycosides, Asterias rollestoni

中图分类号:

樊廷俊,张铮,袁文鹏,丛日山,王卫卫,荆昭 . 水溶性海星皂苷的分离纯化及其抗真菌活性研究[J]. J4, 2008, 43(9): 1-05 .

FAN Ting-jun,ZHANG Zheng,YUAN Wen-peng ,CONG Ri-shan,WANG Wei-wei,JING Zhao . Purification and antifungal activity examination of water-soluble starfish glycosides from Asterias rollestoni[J]. J4, 2008, 43(9): 1-05 .

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