关键词: 青山羊;毛囊;体外培养;培养基;细胞生长因子

Abstract:

In order to set up methods for goat hair follicles cultured in vitro, the regular cross test design L9(34) was used to evaluate the effects of three kinds of mediums with the additron of three differential levels of VEGF and EGF. A hair follicle from the skin of a Jining Grey Goat was separated and cultured in serum-free Williams E, serum-free DMEM and DMEM with 10％ fetal calf serum (FCS), by respectively adding different concentrations of vascular endothelial growth factor (VEGF) and epidermal growth factor(EGF) in every medium. The length of the hair follicle and the average hair shaft elongation (HSE) within the first 8 days were measured by an eyepiece micrometer, and its morphological changes were observed continuously under an invert microscope. The results show that a hair follicle cultured in 9 kinds of mediums grew well, but a hair follicles cultured in serum-free medium grew faster than that in a medium with 10％ serum; the growth rate is low in the medium without cellular growth factors compared with the mediums with the addition of VEGF or／and EGF with 2％～10％ concentration.

Key words: Jining Grey Goat; hair follicle; cultured in vitro; medium; cellular growth factor