Abstract:

The effect of intraperitoneal injection of indigowoad root polysaccharide (IRPS) on the oxidation resistance was studied in the liver of Pelodiscus sinensis. There were four groups in this experiment and every group was continuously injected for three days. The first three groups were injected with 1.5?mg IRPS,3?mg IRPS, and 6?mg IRPS respectively. The fourth group was injected with physiological saline. The results showed that the activities of SOD, CAT and GSHPX increased and MDA content decreased in the first three groups injecting with IRPS. However, there were some differences among them. Compared with the control, CAT activity significantly increased in the first three groups injected with IRPS and the activity increased with an increase of injection dosage. GSHPX activity increased significantly and MDA content obviously decreased by injecting with 3?mg and 6?mg IRPS in  P.sinensis, but the greatest  effect was shown by injecting with 3?mg IRPS in  P.sinensis. The effect on SOD activity was not significant by injecting with 1.5?mg and 3?mg IRPS in  P.sinensis. The effect of the group by injecting with 6?mg IRPS  in  P.sinensis  is the most significant difference. In a word, IRPS could improve the oxidation resistance in the liver of P.sinensis.

Key words: Indigowoad root polysaccharide； P.sinens is； SOD； CAT； GSHPX； MDA