JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2024, Vol. 59 ›› Issue (11): 8-19.doi: 10.6040/j.issn.1671-9352.0.2023.437

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Effects of semisolid-state fermentation of probiotics consortium on release of effective constituents from licorice and their activities

ZHOU Kemeng1, DAI Jianying1, DONG Yuesheng1, DANG Yanyan2, ZHANG Genlin2, XIU Zhilong1*   

  1. 1. School of Bioengineering, Dalian University of Technology, Dalian 110624, Liaoning, China;
    2. School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, Xinjiang, China
  • Published:2024-11-29

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Abstract: The cell wall of licorice prevents the release of effective substances such as flavonoids and polysaccharides from liquorice cells. In this study, a mixed probiotics consortium composed of Saccharomyces cerevisiae, lactic acid bacteria and Bacillus was used in semisolid-sate fermentation of liquorice powder to promote the release of effective substances. The inhibition ability on pathogenic bacteria and tyrosinase as well as antioxidant activities of the fermentation broth were evaluated. The experimental results showed that the extraction rates of polysaccharides, flavonoids, glycyrrhizic acid and glycyrrhetinic acid could be considerably compared with the Soxhlet method. The inhibition rate of the mixed fermentation supernatant on Escherichia coli, Klebsiella pneumoniae and Acinetobacter baumannii was 73.85%, 45.57% and 39.92%, respectively. The scavenging rates of the mixed fermentation supernatant to 1,1-diphenyl-2-picrylhydrazyl(DPPH), 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate(ABTS), 2-Phenyl-4,4,5,5-tetramethylimidazoline-3-oxide-1-oxyl(PTIO), superoxide radical and hydroxyl radical were superior to other extraction methods. The inhibition rate on tyrosinase was 61.62%. The semisolid-state fermentation of licorice by probiotic consortium provides high-quality raw materials for the development of health-care products and cosmetics.

Key words: probiotics, licorice, effective component, antibacterial effect, antioxidant activity, tyrosinase inhibition

CLC Number: 

  • Q939.9
[1] 徐谓,李洪军,贺稚非. 甘草提取物在食品中的应用研究进展[J]. 食品与发酵工业,2016,42(10):274-281. XU Wei, LI Hongjun, HE Zhifei. Research advances in application of licorice extracts as food additives[J]. Food and Fermentation Industries, 2016, 42(10):274-281.
[2] 张耀峰. 甘草及其活性成分的药理活性研究进展[J]. 中医临床研究,2019,11(9):141-142. ZHANG Yaofeng. Research progress on pharmacological activities of Glycyrrhiza uralensis Fisch and its active components[J]. Clinical Journal of Chinese Medicine, 2019, 11(9):141-142.
[3] 李想,李冀. 甘草提取物活性成分药理作用研究进展[J]. 江苏中医药,2019,51(5):81-86. LI Xiang, LI Ji. The research progress of the pharmacological function of active components extracted from licorice[J]. Jiangsu Journal of Traditional Chinese Medicine, 2019, 51(5):81-86.
[4] 李容,陈华国,周欣. 植物多糖免疫调节机制研究进展[J]. 天然产物研究与开发,2018,30(11):2017-2022, 2031. LI Rong, CHEN Huaguo, ZHOU Xin. Immunomodulatory effects of plant polysaccharides:a review of the mechanisms[J]. Natural Product Research and Development, 2018, 30(11):2017-2022, 2031.
[5] 柴美灵,武晓英,张晓红,等. 甘草多糖的免疫调节和抗炎作用机制研究进展[J]. 中国兽药杂志,2021,55(5):66-71. CHAI Meiling, WU Xiaoying, ZHANG Xiaohong, et al. Advances in immunomodulation and anti-inflammatory mechanism of glycyrrhiza polysaccharide[J]. Chinese Journal of Veterinary Drug, 2021, 55(5):66-71.
[6] 郭敏. 甘草多糖降尿酸作用及颗粒剂的研究[D]. 镇江:江苏大学,2018. GUO Min. Study on hypouricemic activities and granules formulation of Glycyrrhiza uralensis polysaccharides[D]. Zhenjiang: Jiangsu University, 2018.
[7] JIANG Y X, DAI Y Y, PAN Y F, et al. Total flavonoids from radix glycyrrhiza exert anti-inflammatory and antitumorigenic effects by inactivating iNOS signaling pathways[J]. Evidence-based Complementary and Alternative Medicine: ECAM, 2018, 2018:6714282.
[8] FRATTARUOLO L, CARULLO G, BRINDISI M, et al. Antioxidant and anti-inflammatory activities of flavanones from glycyrrhiza glabra L.(licorice)leaf phyto complexes: identification of licoflavanone as a modulator of NF-kB/MAPK pathway[J]. Antioxidants, 2019, 8(6):186.
[9] 刘晓钦,邱学军,潘东梅,等. 甘草附子汤的临床应用及药理作用研究进展[J]. 广东药科大学学报,2023,39(2):154-158. LIU Xiaoqin, QIU Xuejun, PAN Dongmei, et al. Research progress of clinical application and pharmacological effect of Gancao fuzi decoction[J]. Journal of Guangdong Pharmaceutical University, 2023, 39(2):154-158.
[10] 王朝霞,宋丽君,杨贵贞. 甘草甜素的研究现状[J]. 中国中西医结合杂志,2002,22(10):796-798. WANG Zhaoxia, SONG Lijun, YANG Guizhen. Current status of study on glycyrrhizin[J]. Chinese Journal of Integrated Traditional and Western Medicine, 2002, 22(10):796-798.
[11] PARK J Y, JI Y J, SEO K H, et al. Heat treatment improves UV photoprotective effects of licorice in human dermal fibroblasts[J]. Processes, 2021, 9(6):1040.
[12] 曹思瑶,宋卓,林鑫宇,等. 乌拉尔甘草不同溶剂提取物的美白活性比较研究[J]. 长春中医药大学学报,2019,35(1):140-143,146. CAO Siyao, SONG Zhuo, LIN Xinyu, et al. Comparative study on whitening activity of different solvent extracts from glycyrrhiza[J]. Journal of Changchun University of Chinese Medicine, 2019, 35(1):140-143, 146.
[13] 印遇龙,杨哲. 天然植物替代饲用促生长抗生素的研究与展望[J]. 饲料工业,2020,41(24):1-7. YIN Yulong, YANG Zhe. Research and prospect of natural plant substitute for antibiotic growth promoters in feed[J]. Feed Industry, 2020, 41(24):1-7.
[14] 曾慧婷,戴迪,何小群,等. 大健康背景下药食两用药渣的资源化利用研究实践与策略[J]. 中国中药杂志,2022,47(14):3968-3976. ZENG Huiting, DAI Di, HE Xiaoqun, et al. Research practice and strategy of resource utilization of residues for edible medicinal plants under background of Big Health[J]. China Journal of Chinese Materia Medica, 2022, 47(14):3968-3976.
[15] 孙悦,何莲芝,苏卓文,等. 超声辅助低共熔溶剂提取甘草多糖的研究[J]. 食品研究与开发,2021,42(2):84-91. SUN Yue, HE Lianzhi, SU Zhuowen, et al. Ultrasonic-assisted eutectic solvent extraction of glycyrrhiza polysaccharide[J]. Food Research and Development, 2021, 42(2):84-91.
[16] 杨嘉丹,刘婷婷,张闪闪,等. 微波辅助提取银耳多糖工艺优化及其流变、凝胶特性[J]. 食品科学,2019,40(14):289-295. YANG Jiadan, LIU Tingting, ZHANG Shanshan, et al. Optimization of microwave-assisted extraction and rheological and gelling properties of polysaccharide from Tremella fuciformis[J]. Food Science, 2019, 40(14):289-295.
[17] 孙晓珊,林冬梅,王铮,等. 甘草及其提取物的药理作用、提取工艺及在家禽养殖中的应用[J]. 饲料研究,2021,44(11):139-141. SUN Xiaoshan, LIN Dongmei, WANG Zheng, et al. Pharmacological function, extraction technology and application of Glycyrrhiza uralensis Fisch and its extract in poultry breeding[J]. Feed Research, 2021, 44(11):139-141.
[18] 刘永岭,王艳艳. 正交设计优选甘草多糖的提取工艺[J]. 价值工程,2018,37(26):249-251. LIU Yongling, WANG Yanyan. Study on extraction of glycyrrhiza polysaccharide orthogonal design[J]. Value Engineering, 2018, 37(26):249-251.
[19] 李晴,申美伦,李翠芹. 甘草多糖提取分离方法的研究进展[J]. 天津中医药大学学报,2020,39(3):250-257. LI Qing, SHEN Meilun, LI Cuiqin. Progress on extraction and separation of polysaccharide from licorice[J]. Journal of Tianjin University of Traditional Chinese Medicine, 2020, 39(3):250-257.
[20] 马彦花,毛福英,赵云生,等. 甘草多糖饮料制备工艺[J]. 食品工业,2019,40(9):12-14. MA Yanhua, MAO Fuying, ZHAO Yunsheng, et al. The preparation technology of the drinks for glycyrrhiza polysaccharide[J]. The Food Industry, 2019, 40(9):12-14.
[21] ZHANG X, MIAO Q, PAN C, et al. Research advances in probiotic fermentation of Chinese herbal medicines [J]. iMeta, 2023, 2(2):2-19.
[22] LIN Y Y, YANG T, SHEN L Y. Study on the properties of Dendrobium officinale fermentation broth as functional raw material of cosmetics[J]. Journal of Cosmetic Dermatology, 2022, 21(3):1216-1223.
[23] KIM S H, YOEM S H, KIM J H, et al. Enhancement of TRP gene expression and UV absorption by bioconverted chestnut inner shell extracts using Lactiplantibacillus plantarum[J]. Molecules, 2022, 27(15):4940.
[24] SHAN C H, GUO J J, SUN X S, et al. Effects of fermented Chinese herbal medicines on milk performance and immune function in late-lactation cows under heat stress conditions[J]. Journal of Animal Science, 2018, 96(10):4444-4457.
[25] DAI J Y, YANG Y, DONG Y S, et al. Solid-state co-cultivation of Bacillus subtilis, Bacillus mucilaginosus, and Paecilomyces lilacinus using tobacco waste residue[J]. Applied Biochemistry and Biotechnology, 2020, 190(3):1092-1105.
[26] ZHANG C L, WANG C, XIU Z L. Regulation of c-di-GMP in biofilm formation of Klebsiella pneumoniae in response to antibiotic and probiotic supernatant in a chemostat system [J]. Current Microbiology, 2021, 78(1):133-143.
[27] 池源,王丽波. 苯酚-硫酸法测定南瓜籽多糖含量的条件优化[J]. 食品与机械,2014,30(1):89-92. CHI Yuan, WANG Libo. Study on phenol-sulfuric acid method for determination of polysaccharide content in pumpkin seeds[J]. Food & Machinery, 2014, 30(1):89-92.
[28] 赵丽娜,王银香,罗锋,等. 三种甘草渣甘草黄酮含量及其抗氧化活性比较研究[J]. 塔里木大学学报,2007,19(4):14-16. ZHAO Lina, WANG Yinxiang, LUO Feng, et al. Content and antioxidant activity of licorice flavoloids from residues of glycyrrhiza[J]. Journal of Tarim University, 2007, 19(4):14-16.
[29] 樊梅娜,王丽荣,张国珍,等. 益生菌发酵甘草对甘草酸含量及其抑菌活性的影响[J]. 中国畜牧杂志,2022,58(10):282-286. FAN Meina, WANG Lirong, ZHANG Guozhen, et al. Effect of Glycyrrhiza uralensis Ficsh fermented by probiotics on glycyrrhizic acid content and its antibacterial activity[J]. Chinese Journal of Animal Science, 2022, 58(10):282-286.
[30] 靳雪飞,何云娇,张杰,等. HPLC法同时测定甘草中3种成分[J]. 中成药,2020,42(10):2786-2788. JIN Xuefei, HE Yunjiao, ZHANG Jie, et al. Simultaneous determination of three components in Glycyrrhiza uralensis Fisch by HPLC[J]. Chinese Traditional Patent Medicine, 2020, 42(10):2786-2788.
[31] 王秋丹,赵凯迪,林长青. 葛根多糖抗氧化性及其降血糖作用研究[J]. 食品工业科技,2022,43(5):381-388. WANG Qiudan, ZHAO Kaidi, LIN Changqing. Study on antioxidant properties of Pueraria lobata polysaccharides and its hypoglycemic effect[J]. Science and Technology of Food Industry, 2022, 43(5):381-388.
[32] SZYDŁOWSKA-CZERNIAK A, ŁASZEWSKA A. Effect of refining process on antioxidant capacity, total phenolics and prooxidants contents in rapeseed oils[J]. Food Science and Technology, 2015, 64(2):853-859.
[33] LI X C. 2-phenyl-4, 4, 5, 5-tetramethylimidazoline-1-oxyl 3-oxide(PTIO·)radical scavenging:a new and simple antioxidant assay in vitro[J]. Journal of Agricultural and Food Chemistry, 2017, 65(30):6288-6297.
[34] WANG C J, ROSENFELDT E, LI Y, et al. External standard calibration method to measure the hydroxyl radical scavenging capacity of water samples[J]. Environmental Science & Technology, 2020, 54(3):1929-1937.
[35] LIUBIMOVSKII S O, USTYNYUK L Y, TIKHONOV A N. Superoxide radical scavenging by sodium 4, 5-dihydroxybenzene-1, 3-disulfonate dissolved in water: experimental and quantum chemical studies[J]. Journal of Molecular Liquids, 2021, 333:115810.
[36] XIA J, LI R Q, HE A Y, et al. Production of poly(β-l-malic acid)by Aureobasidium pullulans HA-4D under solid-state fermentation[J]. Bioresource Technology, 2017, 244:289-295.
[37] KAŠKONIEN(·overE)V, STANKEVICIUS M, BIMBIRAIT(·overE)-SURVILIEN(·overE)K, et al. Current state of purification, isolation and analysis of bacteriocins produced by lactic acid bacteria[J]. Applied Microbiology and Biotechnology, 2017, 101(4):1323-1335.
[38] CHAVES-LÓPEZ C, SERIO A, GIANOTTI A, et al. Diversity of food-borne Bacillus volatile compounds and influence on fungal growth[J]. Journal of Applied Microbiology, 2015, 119(2):487-499.
[39] 郭如意,谭剑,肖嘉琦,等. 混菌固态培养生产玉米皮发酵饲料工艺条件的研究[J]. 饲料研究,2021,44(20):53-59. GUO Ruyi, TAN Jian, XIAO Jiaqi, et al. Solid-state fermentation of corn bran with mixed probiotics for bio-feed production[J]. Feed Research, 2021, 44(20):53-59.
[40] LENCOVA S, ZDENKOVA K, DEMNEROVA K, et al. Antibacterial and antibiofilm effect of natural substances and their mixtures over Listeria monocytogenes, Staphylococcus Aureus and Escherichia coli[J]. LWT, 2022, 154:112777.
[41] RUDRAPAL M, KHAIRNAR S J, KHAN J, et al. Dietary polyphenols and their role in oxidative stress-induced human diseases: insights into protective effects, antioxidant potentials and mechanism(s)of action[J]. Frontiers in Pharmacology, 2022, 13:806470.
[42] ULLAH H, HUSSAIN Y, SANTARCANGELO C, et al. Natural polyphenols for the preservation of meat and dairy products[J]. Molecules, 2022, 27(6):1906.
[43] 张智萍. 川芎美白活性成分的提取分离及在化妆品中的应用研究[D]. 广州:广东药学院, 2014. ZHANG Zhiping. Extraction and separation skin-whitening agent from rhizoma Chuanxiong and its application in cosmetics[D]. Guangzhou: Guangdong Pharmaceutical University, 2014.
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