JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2024, Vol. 59 ›› Issue (11): 40-50.doi: 10.6040/j.issn.1671-9352.0.2023.488

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Staining methods on arbuscular mycorrhizal fungi in Lycium barbarum roots and the relationship between colonization rate and soil factors

YANG Mu1,2,3, JI Shenglian1,2,3, GUO Huan1,2,3, DUAN Guozhen1,3*, FAN Guanghui1,3, LI Jianling1,3, WANG Zhanlin1,3   

  1. 1. Academy of Agricultural and Forestry Sciences, Qinghai University, Xining 810016, Qinghai, China;
    2. College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, Qinghai, China;
    3. Qinghai Plateau Key Laboratory of Tree Genetics and Breeding, Xining 810016, Qinghai, China
  • Published:2024-11-29

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Abstract: Understanding the infection and colonization of arbuscular mycorrhizal fungi(AMF)is the basis for mobilizing indigenous AMF to improve plant resistance. Lycium barbarum in nine fields of the Qaidam Basin were the subject. Based on the basic staining method of AMF, previous studies were optimised to investigate the main factors influencing the observation of AMF on the roots of perennial field-grown L.barbarum. The influence of different regions, soil pH, soil physicochemical properties and the colonization rate of AMF on L.barbarum were also compared. The results showed that the optimal observation effect of AMF colonization rate on the roots of Perennial field-grown L.barbarum in the Qaidam Basin was achieved by the following steps. First, the fixed root segments were placed in a 10% KOH 90 ℃ for 90 min, then in the boiling alkaline peroxide(3 mL NaOH+30 mL 10% H2O2+H2O to 600 mL)for 15-20 min, followed by 5 min of H2O2 bleaching, 5 min of lactic acidification, 5 min of acetone ink staining and 30 min of lactic acid glycerol 90 ℃ decolourisation. This method can clearly observe the structures of vesicles and hyphae in the roots of perennial field-grown L.barbarum. The average AMF colonization rate of perennial field-grown L.barbarum in the Qaidam Basin was about 41.32%. RDA analysis showed that the AMF colonization rate in L.barbarum roots was mainly affected by factors such as soil pH, available phosphorous content(xAP), and available potassium content(xAK), among which the soil pH showed a significant positive correlation with the AMF colonization rate, while xAP and xAK showed a significant negative correlation with the colonization rate.

Key words: Lycium barbarum, arbuscular mycorrhizal fungi, colonization rate, redundancy analysis

CLC Number: 

  • Q948.12
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