JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2025, Vol. 60 ›› Issue (9): 24-30.doi: 10.6040/j.issn.1671-9352.0.2025.115

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Preparation of Acorus tatarinowii volatile oil nasal thermosensitive in situ gel

CHEN Liang1, DU Lilin2, XIAO Yaxin1, LI Jiaxin1*   

  1. 1. College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, China;
    2. The First Affiliated Hospital, Guizhou University of Traditional Chinese Medicine, Guiyang 550001, Guizhou, China
  • Published:2025-09-10

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Abstract: This study successfully prepared a nasal thermosensitive in situ gel of Acorus tatarinowii volatile oil. The complexation process parameters of Acorus tatarinowii volatile oil were optimized using orthogonal design method. Meanwhile, taking the mass fractions of poloxamer 407(P407)and poloxamer 188(P188)as the key factors and phase transition temperature as the index, the binomial fitting was carried out using central composite design-response surface methodology. Based on this, the preparation process of the nasal thermosensitive in situ gel was further optimized, and its inhibitory activity against γ-aminobutyric acid transaminase(GABA-T)was assessed. The results indicated that the optimal inclusion complexation process parameters for Acorus tatarinowii volatile oil were as follows: the feeding ratio of volatile oil to β-cyclodextrin(β-CD)was 1 mL∶5 g, the feeding ratio of β-CD to H2O was 1 g∶35 mL, and the complexation temperature was 30 ℃. When the mass fraction of P407 was 19.13% and the mass fraction of P188 was 1.08%, the phase transition temperature of the prepared in situ gel was 32 ℃, which had a promising application prospect. Moreover, the gel exhibited a dose-dependent inhibitory effect on GABA-T, with an IC50 value of 215.7 μg/mL. The thermosensitive nasal in situ gel preparation process established in this study was efficient and feasible, providing a solid theoretical basis and technical support for the development of Acorus tatarinowii volatile oil and its application in the treatment of nervous system diseases.

Key words: Acorus tatarinowii Schott, central composite design-response surface methodology, in situ gel, volatile oil

CLC Number: 

  • R285
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[1] Liang CHEN, Chengwei FANG, Ji MENG, Xiuping MA, Dan ZHU, Jiaxin LI. Mechanism of Acotus tatarinowii Schott in treating Alzheimer's disease [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2024, 59(11): 64-73, 92.
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