Abstract:

A simple and sensitive new method for the determination of total flavonoids in ginkgo biloba extract was put forth. The experiment results indicated that the fluorescence intensity of ginkgo biloba extract (GBE) was greatly enhanced by heating in an acid medium and forming complex with Al3+. A fluorimetric method for determination of total flavonoids in GBE was proposed by taking quercetin as standard sample. Under the optimum conditions, a linear relationship was obtained between the fluorescence intensity and the concentration of quercetin in the range of 0000?75～050?μg／mL(R=0999?9; P<0000?1), and the detection limit was 045?ng／mL(S／N=3). This method was applied for determining the total flavonoids in GBE, the recovery test was put forth by standard addition method, and the satisfied results were obtained.

Key words: spectrofluorimetry; quercetin; ginkgo biloba extract; total flavonoids