Abstract:

The main focuses of this thesis are evaluate and screening of the growth of 12 Elm Clones under different concentration of NaCl (0.3%,0.4%,0.5%,0.6%,0.7%,0.8%) in Vitro Culture. The impact of changes in the external environment and conditions on clone salt-tolerance of Ulmus pumila was excluded. By using the method of membership function, the ability of NaCl tolerance for the 12 elm clones has analyzed and evaluated. The result shows that No.58, No.30 and No.50 elm clones have strong resistance, also the result from principal component analysis are similarity; at the same time, the result of principal component analysis (PCA) divides the 12 Elm Clones into 3 classes according to the application situation, which provide a reliable evaluation methodology for the tolerance of elm clones toward NaCl.

Key words: Ulmus pumila clones; tissue culture; salt tolerance