Abstract: In vitro transcription of RNA synthesized DjRock2 gene probe, the detection efficiency of RNA probe, and regeneration of adult planarians in situ hybridization. Then insert DjRock2 gene to the vector L4440, construct L4440-DjRock2 interference vector, synthetic dsRNA and microinjection planarian, using RT-PCR and Western blot technique to detect interference regeneration process expressing down effect on DjRock2 mRNA and protein. The results showed that: DjRock2 genes specifically expressed in mature planarian central nervous system regeneration blastema wound position has a positive signal; interfere adult planarian regeneration of the first three days at the head, middle and tail of the embryo group has, but the first five days at the beginning of the wound apoptosis, adult stem cells do not complete the normal differentiation of the head, middle and tail regeneration is inhibited, not the normal completion of regeneration, and even death. RT-PCR technique significantly decreased DjRock2 mRNA expression was detected after RNA interference. Upon detection of interference Western blot protein expression DjRock2 down. Constructed L4440-DjRock2 high interference carrier interference efficiency, phenotypic changes significantly, the analysis of gene and protein, laid the foundation for further research Rock2 gene function.

Key words: DjRock2, RNAi, regeneration, RT-PCR, Dugesia japonica