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Table of Content

      
    16 May 2008
    Volume 43 Issue 5
    Articles
    Influences of different mediums on goat hair follicles cultured in vitro
    CUI Zhi-feng,ZHAO Jing*,WANG Hui*,HAO Zhi-ru,ZHANG Zhao-yuan
    J4. 2008, 43(5):  1-05 .  doi:
    Abstract ( 1203 )   PDF (448KB) ( 1134 )   Save
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    In order to set up methods for goat hair follicles cultured in vitro, the regular cross test design L9(34) was used to evaluate the effects of three kinds of mediums with the additron of three differential levels of VEGF and EGF. A hair follicle from the skin of a Jining Grey Goat was separated and cultured in serum-free Williams E, serum-free DMEM and DMEM with 10% fetal calf serum (FCS), by respectively adding different concentrations of vascular endothelial growth factor (VEGF) and epidermal growth factor(EGF) in every medium. The length of the hair follicle and the average hair shaft elongation (HSE) within the first 8 days were measured by an eyepiece micrometer, and its morphological changes were observed continuously under an invert microscope. The results show that a hair follicle cultured in 9 kinds of mediums grew well, but a hair follicles cultured in serum-free medium grew faster than that in a medium with 10% serum; the growth rate is low in the medium without cellular growth factors compared with the mediums with the addition of VEGF or/and EGF with 2%~10% concentration.

    The establishment of a technique system for differential proteomics of body fluids
    QIN Zhao-yu,ZHANG Xiao-kui ,HE Feng,LIU Shi-lian*
    J4. 2008, 43(5):  6-09 .  doi:
    Abstract ( 892 )   PDF (650KB) ( 1043 )   Save
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    In order to establish a technique system for differential proteomics of body fluids, cerebrospinal fluid samples in two different physiological states were studied. There was a Multile Sclerosis sample and a control for each group, which was done in triplicate. Samples were precipitated with ice-cold acetone and the protein concentration was accurately quantified. The control and abnormal groups were labeled with two different Cyanine Dyes (Cy2 and Cy5) using the minimal labeling method while the internal standard group was labeled with Cy3. Then 2D-E was used and three gels were run. Each gel contained a control group, an abnormal group and the internal standard group labeled with 3 different Cye Dyes. The information including differential express protein spots and their levels were obtained using a Typhoon 9400 scanner and DeCyder 2-D software. Protein spots were identified by MALDI-TOF/TOF. Metacore integrated software was used to analyze the interactions between proteins. The results show that a differential proteomics technique system is effective to discover valuable candidate proteins.

    Genetic polymorphisms of the prolactin gene A8398G and its correlation with milk production traits in Chinese Holstein cows

    WANG Li-juan..LI Qiu-ling,WANG Hong-mei,LI Jian-bin,WANG Zhi-yu,WANG Chang-fa,HOU Ming-hai,ZHONG Ji-feng*

    J4. 2008, 43(5):  10-13 .  doi:
    Abstract ( 918 )   PDF (572KB) ( 932 )   Save
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    Single nucleotide polymorphisms of exon 4 A8398G of the PRL gene were detected in Chinese Holstein cows by PCR-RFLP. The results showed that the PRL gene digested by RsaⅠ could be divided into two kinds of alleles and three kinds of genotypes. The frequencies of allele A and G are 0.257 and 0.743 respectively.The frequencies of genotype AA, AG and GG are 0.010, 0.497 and 0.493 respectively. Sequencing revealed one single nucleotide mutatioo A→G at 8398 bp of the gene in genotype AA in comparison with genotype GG, and this mutation can not result in an amino acid change. The result of Chi Square test indicated that the frequencies of patterns types of PRL gene genes digested by RsaⅠ can not fit with the Hardy-Weinberg equilibrium in this population(P<0.05). In lactation Ⅱ, the least squares means of protein percentage of this population was higher for genotype GG than for genotype AG (P<0.05). The least squares means of milk yield of this population was higher for genotype AG than for genotype GG (P<0.01). The least squares means of linear score of this population was higher for genotype GG than for genotype AG (P<0.05).

    The Monte Carlo study of W boson polarization
    LIU Ming-hui,ZHU Cheng-guang
    J4. 2008, 43(5):  14-18 .  doi:
    Abstract ( 769 )   PDF (755KB) ( 772 )   Save
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    In t dileptonic decay channel, a Monte Carlo data sample with the integral luminosity about 0.7fb-1 was used to study the W boson polarization by measuring the angle distributions of the final particles from W boson. The result was: F0=0.709±0.066(syst)±0.044(stat), FL=0.297±0.077(syst)±0.026(stat), FR=-0.005±0.033(syst)±0.027(stat)。

    Multi-scale image analysis applied to distinguish primary iron of cosmic rays in the knee region
    QU Xiao-bo,FENG Cun-feng,ZHANG Xue-yao,XUE Liang
    J4. 2008, 43(5):  19-23 .  doi:
    Abstract ( 1125 )   PDF (621KB) ( 816 )   Save
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    Based on the Monte Carlo simulation for ARGO-YBJ experiment, the characteristics of lateral distribution of the charged particles in Extensive Air Showers induced by primary cosmic ray particles with energy at the “knee” region were analyzed by using the multi-fractal and discrete wavelet methods under different length scales. Parameters, which can characterize the difference between different primary particles, were found. With these parameters as the inputs, an artificial neural network was created for multi-variant analysis, and the discrimination power was given.

    Theoretical study of electron paramagnetic resonance of Cr3+ ions in AlBr3·6H2O crystals
    WANG Min-jie,ZHU Lian-xuan*,LIN Ai-ying
    J4. 2008, 43(5):  24-26 .  doi:
    Abstract ( 1127 )   PDF (223KB) ( 710 )   Save
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    According to the semi-SCF d-orbit radial function, the point-charge model and the third-order perturbation formulae, the g-factors(g=1.9707) and the zero-field splitting D(D=-0.0323) were calculated, and the calculated results are in good agreement with the experimental results(experimental values g=1.976±0.001, D=-0.032?5±0.0001). The bond length R=(Cr3+—O2+)=0.191nm is rational. The angle increases a little (the increment  0.35°) after the Cr3+ replace the Al3+in AlBr3·6H2O crystals.

    The effects of bias voltage and the distance between two electrodes on the transport properties of the Au/molecule/Au junctions
    XUAN Shu-ke
    J4. 2008, 43(5):  27-31 .  doi:
    Abstract ( 831 )   PDF (470KB) ( 762 )   Save
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    Based on the first principle calculation method, the effects of bias voltage and the distance between two electrodes on the transport properties of the Au/molecule/Au junctions was investigated. The bias voltage corresponding to the first onset of the conductance decreases with the electrode distance from 1.278nm to 1.298nm, and increases with the electrode distance from 1.298nm to 1.398nm. The electron accumulation at the interface between molecule and electrode resulting from the bias can hamper electron tunneling. Good agreement between calculation results and experimental results in conductive features was achieved.

    On para-communication and pan-communication (Ⅱ)
    CHEN Lei
    J4. 2008, 43(5):  32-38 .  doi:
    Abstract ( 1262 )   PDF (294KB) ( 880 )   Save
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    Based on the statistical models of synchronous and asynchronous communication, the concept and structure of a communication decision tree were introduced. From the observation of human communication behavior, the pan-communication law was induced. Some important concepts, such as comtropy function, mean comtropy, information amount of communication sessions and communication effectiveness were proposed.

    Sub-stroke extraction research on the off-line hand-written recognition of Chinese characters
    JIN Tian-fei,
    J4. 2008, 43(5):  39-44 .  doi:
    Abstract ( 801 )   PDF (662KB) ( 886 )   Save
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    Based on the present realizing method of thinning and feature point extraction, an improved thinning algorithm based on groups and a far inflection-point method was proposed. The method of the improved thinning algorithm can mark the type of branch points in the character images, which is based on group numbers in the thinning process. This method can prepare for sequential sub-stroke extraction. The far inflection-point can provide the method, which could quickly extract a Chinese character inflection-point. Experimental results show that these proposed algorithms can fairly well extract sub-strokes, and the feature point extraction accuracy is 98.6%.

    White noise functional solutions of a Wick-type stochastic KdV-MKdV equation
    ZHU Hong ,LIU Xiong
    J4. 2008, 43(5):  45-49 .  doi:
    Abstract ( 873 )   PDF (253KB) ( 661 )   Save
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    The basic theory of utilizing Almeter transformation to get the exact solution to Wick-typed stochastic general KdV-MKdV equations set is as follows: first, the Wick-typed stochastic general KdV-MKdV equations set was changed into general coefficient KdV equations set by Almeter transformation; then, the exact solution to the KdV equations set was obtained by the methods of a special transformation; finally, the exact solution to the original equations set was obtained by the Almeter inverse transformation.

    The existence of multiple positive solutions for higher order boundary value systems with p-Laplacian operator
    TANG Qiu-yun,WANG Ming-gao,LIU Yan-sheng
    J4. 2008, 43(5):  50-53 .  doi:
    Abstract ( 998 )   PDF (192KB) ( 912 )   Save
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    With the method of compression and expansion of a cone, the existence of multiple positive solutions for higher order boundary value systems with a p-Laplacian operator was investigated.

    Global convergence of a conjugate gradient method with strong Wolfe-Powell line search
    LIU Li-ying,LI Ying ,
    J4. 2008, 43(5):  54-57 .  doi:
    Abstract ( 909 )   PDF (198KB) ( 1048 )   Save
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    A new kind of conjugate gradient algorithm for solving nonlinear unconstrained optimization was proposed. This conjugate gradient method with strong Wolfe-Powell line search satisfies the sufficient descent property, and this new kind of conjugate gradient algorithm with strong Wolfe line search possess the global convergence property.

    Indefinite stochastic LQ problem with exponential stability degree constraint
    MA Hong-ji,HOU Ting,XING Jian-min
    J4. 2008, 43(5):  58-62 .  doi:
    Abstract ( 781 )   PDF (229KB) ( 467 )   Save
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    An indefinite stochastic LQ problem was studied in an infinite time horizon with stability degree constraint. The objective was to find an optimal control, by which the cost function minimum and the state with the optimal control having a higher mean-square convergence rate can be obtained. First, a necessary and sufficient condition was provided for the well-posedness of this problem. Then, a sufficient condition for the attainability was obtained based on the solvability of a generalized algebraic Riccati equation.

    The Dα-derived set in LF topological space
    SUN Shou-bin,MENG Guang-wu
    J4. 2008, 43(5):  63-65 .  doi:
    Abstract ( 748 )   PDF (171KB) ( 751 )   Save
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    In L-fuzzy topological space, the concept of Dα-derived set was defined based on Dα-closed sets. The characteristics of the concept were systematically discussed.

    Methods of lower and upper solutions and the solvability of a three-point boundary value problem at resonance
    XU Ling
    J4. 2008, 43(5):  66-70 .  doi:
    Abstract ( 813 )   PDF (211KB) ( 639 )   Save
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    The methods of lower and upper solutions for a second order three-point boundary value problem at resonance
    u″(t)=f(t, u(t), u′(t)), t∈[0, 1],
    u′(0)=0, u(1)=u(η)
    were developed by using the connectivity properties of the solution sets of parameterized families of compact vector fields,
    where η∈(0, 1), f:[0, 1]×R2→R is continuous and satisfies the Nagumo condition.

    Relations of generalized invariant monotonicity and invexity
    LI Ai-qin,FAN Li-ya
    J4. 2008, 43(5):  71-74 .  doi:
    Abstract ( 764 )   PDF (148KB) ( 435 )   Save
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    The relationships between generalized invexity of locally Lipschitz functions and generalized invariant monotonicity of corresponding limiting subdifferentials were studied. The results obtained can be viewed as an improvement of the previous results for non-smooth convex functions.

    The repairable priority queuing system of MX11,MX22/G(M/M)/1 with optional service
    ZHANG Feng
    J4. 2008, 43(5):  75-81 .  doi:
    Abstract ( 782 )   PDF (257KB) ( 934 )   Save
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    A repairable priority MX11,MX22/G(M/M)/1 queuing system with optional service was studied. There are two kinds of customers, in which one must be served first. Both of these two kind customers arrived in bulk and the server can provide two kinds service. After the first phase service is finished, the customers will receive the second service with probability r or leave the system with probability 1-r. The length and some reliability indexes were obtained by the method of supplementary variable.

    Fixed point theorems for the generalized contractive mappings and mapping pairs in cone metric spaces
    YUAN Qing,GAO Jian-jun,WANG Yan-wei
    J4. 2008, 43(5):  82-86 .  doi:
    Abstract ( 1830 )   PDF (200KB) ( 714 )   Save
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    Since the notation of cone metric spaces was raised, several authors have studied its constructions and properties. The results on the existence of fixed points of generalized contractive mappings were extended by relaxing the contractive conditions. The existence of common fixed points of generalized contractive mapping pairs was also discussed. The obtained results extend the recent relative results and the classical theorems in metric spaces.

    Vertex-disjoint 4-cycle in a bipartite graph
    GENG Jian-yan,YAN Jin,LI Feng
    J4. 2008, 43(5):  87-92 .  doi:
    Abstract ( 896 )   PDF (221KB) ( 891 )   Save
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    A set of sub-graphs of G is said to be independent or vertex-disjoint if no two of them have a common vertex in G. It gives the minimum degree condition for a bipartite graph containing k independent 4-cycle, each of which contains a previously specified vertex.

    A condition of isolated toughness on the existence of fractional [a,b]-factors of graphs
    PAN Rui-xia,LAN Mei,LIU Gui-zhen
    J4. 2008, 43(5):  93-96 .  doi:
    Abstract ( 1003 )   PDF (199KB) ( 995 )   Save
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    The relationships between the isolated toughness I(G) and the existence of fractional [a,b]-factors were given. It was proved thatif δ(G)≥I(G)≥a-1+(a-1)/b, then G has a fractional [a,b]-factor where 2≤a<b. The results are best possible in some sense. In addition a conjecture about a=b was given.