Slimb通过泛素化降解hippo信号通路的辅转录因子Yki

doi:10.6040/j.issn.1671-9352.0.2014.412

山东大学学报（理学版） ›› 2015, Vol. 50 ›› Issue (01): 37-41.doi: 10.6040/j.issn.1671-9352.0.2014.412

Slimb通过泛素化降解hippo信号通路的辅转录因子Yki

郭纪伟, 金丹

南开大学生命科学学院, 天津 300071

收稿日期:2014-09-17 修回日期:2014-11-07 出版日期:2015-01-20 发布日期:2015-01-24
作者简介:郭纪伟(1984-),男,博士,研究方向为Hippo信号通路调控器官大小的分子机制.E-mail:guojiwei0510@163.com

Degradation of co-activator Yki of the hippo pathway through ubiquitinationby Slimb

GUO JI-wei, JIN Dan

School of Life Sciences, Nankai University, Tianjin 300071, China

Received:2014-09-17 Revised:2014-11-07 Online:2015-01-20 Published:2015-01-24

摘要/Abstract

摘要： Yki是hippo信号通路中主要的基因转录辅助因子,调节下游靶基因的表达。它在细胞生长分化、组织器官形成以及成体干细胞维持等方面都起着重要的作用。在果蝇中,Slimb是SCF E3泛素连接复合物中的亚基,具有底物专一性,特异性地降解蛋白质。本研究中发现,在体外Slimb能够通过泛素化降解Yki。进一步利用免疫共沉淀的方法,发现Slimb与Yki能够相互作用。果蝇中过表达yki产生的表型能够被同时过表达Slimb所恢复,被Slimb沉默所加剧。这些研究结果提示Slimb作为E3连接酶的一个亚基,可以调节Yki的稳定性,在研究hippo信号通路调节组织生长发育中具有重要的意义。

关键词: hippo信号通路, 降解, yki, slimb, 泛素化

Abstract: Yki, an important co-transcription factor, regulates the expression of downstream target genes of the hippo pathway. Yki is known to be important for development, growth, organogenesis and maintenance of adult stem cells. Slimb as a substrate specificity subunit of the SCF E3 ubiquitin ligase promotes proteins for degradation in the drosophila. In this paper, we found that Slimb degraded Yki by the ubiquitination and the co-immunoprecipitation indicated that Slimb interacted with Yki in vitro. Furthermore, we found that co-overexpression with Slimb caused mostly suppressed Yki wing phenotype and knockdown of Slimb enhanced overexpression with Yki eye phenotype. These results suggested that Slimb as a subunit of the E3 ubiquitin ligase regulated the stability of Yki and provided an important significance in the regulating tissue growth and development of hippo pathway.

Key words: hippo pathway, yki, ubiquitination, degradation, slimb

中图分类号:

Q257

郭纪伟, 金丹. Slimb通过泛素化降解hippo信号通路的辅转录因子Yki[J]. 山东大学学报（理学版）, 2015, 50(01): 37-41.

GUO JI-wei, JIN Dan. Degradation of co-activator Yki of the hippo pathway through ubiquitinationby Slimb[J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2015, 50(01): 37-41.

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Slimb通过泛素化降解hippo信号通路的辅转录因子Yki

Degradation of co-activator Yki of the hippo pathway through ubiquitinationby Slimb

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