一种基于小分子多肽配体检测AD7c-NTP的新方法

doi:10.6040/j.issn.1671-9352.0.2016.119

山东大学学报（理学版） ›› 2016, Vol. 51 ›› Issue (7): 121-125.doi: 10.6040/j.issn.1671-9352.0.2016.119

一种基于小分子多肽配体检测AD7c-NTP的新方法

张燕^1,2,韦宇平¹,张良^1,2,付彦凯^1,2,徐建栋¹,徐霞^1*

1.中国科学院过程工程研究所生化工程国家重点实验室, 北京 100190;2.中国科学院大学, 北京 100049

收稿日期:2016-03-23 出版日期:2016-07-20 发布日期:2016-07-27
通讯作者: 徐霞(1969— ),女,研究员,博士生导师,研究方向为功能多肽和组织工程技术研究. E-mail:xuxia@ipe.ac.cn E-mail:zhangeom@126.com
作者简介:张燕(1990— ),女,硕士研究生,研究方向为体外治疗技术. E-mail:zhangeom@126.com
基金资助:
国家自然科学基金资助项目(21506230)

A novel small peptide ligand for detection of Alzheimer-associated neuronal thread protein

ZHANG Yan^1,2, WEI Yu-ping¹, ZHANG Liang^1,2, FU Yan-kai^1,2, XU Jian-dong¹, XU Xia^1*

1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2016-03-23 Online:2016-07-20 Published:2016-07-27

摘要/Abstract

摘要： 与阿尔兹海默症相关的神经纤维蛋白(AD7c-NTP)是目前被广泛关注的一种用于早期诊断阿尔兹海默症的标志物,该标志物具有易获得、无创性的检测优势。本研究目的是检测尿液中的阿尔兹海默症标志物AD7c-NTP。通过分子模拟设计小分子多肽配体,固相合成方法合成多肽,基因克隆、原核表达AD7c-NTP,采用色谱方法分析小分子多肽配体的吸附能力、AD7c-NTP的检测范围以及在模拟尿液中的吸附情况。研究设计的DEWH配体可以与AD7c-NTP特异性结合,并且结合率达到了85%,该方法检测的线性范围是200~900 μg/L。该研究提供了一种便携的、可重复检测AD7c-NTP的方法,为阿尔兹海默早期诊断及延缓疾病奠定基础。

关键词: 阿尔兹海默症, AD7c-NTP, 亲和肽

Abstract: Alzheimer-associated neuronal thread protein(AD7c-NTP)as a sensitive, easily obtained and noninvasive biomarker for AD disease detection. This research is aim to detect AD7c-NTP protein. A novel peptide for detecting AD7c-NTP was developed using molecular simulations and the peptide was synthesized. The recombinant protein was expressed. The bound protein was tested using chromatography and the mimic urine was detected as well. The peptide of DEWH is able to bind AD7c-NTP with the adsorption rate of around 85% and the linearity range is 200-900 μg/L. This novel peptide could provide an easy and repeatable method for detecting AD disease, which is critical for early diagnosis and postponing disease progression with proper treatment.

Key words: Alzheimer disease, AD7c-NTP, Affinity peptide

中图分类号:

O629.72

张燕,韦宇平,张良,付彦凯,徐建栋,徐霞. 一种基于小分子多肽配体检测AD7c-NTP的新方法[J]. 山东大学学报（理学版）, 2016, 51(7): 121-125.

ZHANG Yan, WEI Yu-ping, ZHANG Liang, FU Yan-kai, XU Jian-dong, XU Xia. A novel small peptide ligand for detection of Alzheimer-associated neuronal thread protein[J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2016, 51(7): 121-125.

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一种基于小分子多肽配体检测AD7c-NTP的新方法

A novel small peptide ligand for detection of Alzheimer-associated neuronal thread protein

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