NaCl胁迫下大毒滑锈伞菌对金银花生长及生理特性的响应

doi:10.6040/j.issn.1671-9352.0.2023.116

摘要/Abstract

摘要：

以金银花(Lonicera japonica)和大毒滑锈伞菌(Hebeloma crustuliniforme)为材料，采用0、60、90、120 mmol/L共4个NaCl浓度的盐胁迫处理，研究了NaCl胁迫下植株根系的大毒滑锈伞菌定殖规律，探明了大毒滑锈伞菌对金银花盆栽苗生长及生理特性的作用机制。结果表明，随着盐浓度的升高，接菌组的脯氨酸含量比对照组的分别高35.98%、141.28%、71.66%、8.79%，且在90、120 mmol/L盐浓度下，大毒滑锈伞菌提高了金银花植株的耐盐性。经相关性和双因素方差分析发现，新梢生长量、分枝增长数与生物量呈显著正相关，脯氨酸与分枝增长数呈极显著负相关。盐胁迫下接种大毒滑锈伞菌能显著降低宿主苗高生长量和显著提高宿主脯氨酸含量，使金银花幼苗通过大量积累脯氨酸来抵御盐分胁迫。

关键词: 大毒滑锈伞菌, 金银花, 菌根真菌, 生理特性, 耐盐性

Abstract:

Lonicera japonica and Hebeloma crustuliniforme were used as materials in this study. 0, 60, 90 and 120 mmol/L NaCl salt treatments were taken out to study the rule of H. crustuliniforme colonization in plant roots and analyze the mechanism of H. crustuliniforme on the growth and physiological characteristics of L. japonica seedlings. The results showed that with the increase in salt concentration, the proline content in the inoculated group plants was 35.98%, 141.28%, 71.66% and 8.79% higher than that of the control group, respectively. Under the treatment of 90 and 120 mml/L salt concentration, the salt tolerance of L.japonica plants was improved by the H. crustuliniforme. The results of correlation and two-way ANOVA indicated thatnew shoot growth and the number of branch growth were positively correlated with the biomass, and proline showed a highly significant negative correlation with the number of branch growth. H. crustuliniforme could significantly reduce the host seedling height growth and increase the host proline content significantly. The inoculation with H. crustuliniforme treatment could increase the proline accumulation of L. japonica seedlings to resist salt adversity under salt stress.

Key words: Hebeloma crustuliniforme, Lonicera japonica, mycorrhizal fungi, physiological characteristics, salt resistance

中图分类号:

S793.9

慕德宇,李楠,王涛,刘瑾,慕宗昭,张靖川. NaCl胁迫下大毒滑锈伞菌对金银花生长及生理特性的响应[J]. 《山东大学学报(理学版)》, 2024, 59(1): 139-150.

Deyu MU,Nan LI,Tao WANG,Jin LIU,Zongzhao MU,Jingchuan ZHANG. Growth and physiological mechanism study of H. crustuliniforme and Lonicera japonica symbiotic relationship under salt tolerance[J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2024, 59(1): 139-150.

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编号	处理浓度/(mmol/L)	侵染率平均值/%
1	0	66.7±7.2
2	60	70.0±4.7
3	90	66.7±2.7
4	120	63.3±7.2

处理	苗高增长量	新梢生长量	分枝增长数	总鲜质量	总干质量	地下鲜质量	地上鲜质量	地下干质量	地上干重
接菌	10.302^*	381.306	3.025	0.091	0.482	0.441	0.132	0.027	0.279
盐胁迫	2.772	23.156	4.292	18.534^*	12.004^*	2.783	8.809^*	0.084	10.322^*
接菌+盐胁迫	2.336	456.740^*	5.292	3.666	6.385	0.701	7.005^*	0.008	6.687

处理	叶绿素a	叶绿素b	叶绿素	相对含水量	脯氨酸含量	可溶性蛋白含量
接菌	0.053	0.000	0.063	0.001	32.581^**	2.493E-05
盐胁迫	0.245	0.048	0.468	10.273^**	26.689^**	0.000
接菌+盐胁迫	0.073	0.029	0.191	3.405^*	5.180	0.001

指标	苗高生长量	新梢生长量	分枝增长数	总鲜质量	总干质量	地下鲜质量	地上鲜质量	地下干质量	地上干质量	叶绿素a含量	叶绿素b含量	总叶绿素含量	相对含水量	脯氨酸含量	可溶性蛋白含量
苗高生长量	1
新梢生长量	0.153	1
分枝增长数	-0.048	-0.182	1
总鲜质量	0.104	0.277	0.305	1
总干质量	0.155	0.342^*	0.325^*	0.959^**	1
地下鲜质量	-0.071	0.024	0.148	0.704^**	0.489^**	1
地上鲜质量	0.183	0.355^*	0.316^*	0.898^**	0.977^**	0.320^*	1
地下干质量	-0.015	0.143	0.212	0.746^**	0.635^**	0.875^**	0.453^**
地上干质量	0.169	0.350^**	0.323^*	0.936^**	0.995^**	0.413^**	0.993^**	0.554^**	1
叶绿素a含量	0.408^*	0.168	0.101	-0.329	-0.280	-0.285	-0.290	-0.138	-0.286	1
叶绿素b含量	0.168	0.237	0.007	-0.187	-0.126	-0.276	-0.076	-0.218	-0.104	0.677^**	1
总叶绿素含量	0.358	0.218	0.076	-0.306	-0.249	-0.304	-0.239	-0.176	-0.246	0.967^**	0.841^**	1
叶片相对含水量	0.096	-0.391	0.299	0.318	0.250	0.326	0.240	0.177	0.247	-0.336	-0.356	-0.369	1
脯氨酸含量	-0.157	0.297	-0.527^**	-0.381	-0.328	-0.352	-0.318	-0.227	-0.326	0.119	0.292	0.188	-0.409^*	1
可溶性蛋白	-0.314	0.049	-0.245	0.048	0.060	0.072	0.019	0.154	0.042	-0.060	-0.214	-0.117	-0.228	-0.094	1

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