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房干村不同植被下可培养细菌多样性研

周娟1,2,郭卫华1,宗美娟1,韩雪梅1,王仁卿1   

  1. 1. 山东大学生命科学学院生态与生物多样性研究所, 山东济南250100;2. 济南军区疾病预防控制中心, 山东济南250014
  • 收稿日期:2006-05-10 修回日期:1900-01-01 出版日期:2006-10-24 发布日期:2006-10-24
  • 通讯作者: 周娟

Analysis of the soil cultivable bacterial diversities underdifferent vegetations of Fanggan village

ZHOU Juan,GUO Wei-hua,ZONG Mei-juan,HAN Xue-mei,WANG REN-qing   

  1. 1. Institute of Ecology and Biodiversity, College of Life Science, Shangdong Univ., Jinan 250100, Shandong, China;2. The Center for Disease Control and Prevention of Jinan Military Commond, Jinan 250014, Shandong, China
  • Received:2006-05-10 Revised:1900-01-01 Online:2006-10-24 Published:2006-10-24
  • Contact: ZHOU Juan

摘要: 利用传统的稀释平板法对房干村不同植被下可培养的异养型好氧性细菌多样性从数量、种类、形态三方面进行动态研究,并利用BIOLOG鉴定系统结合显微观察对部分优势菌株进行了鉴定.结果显示不同植被下可培养好氧性异养型细菌多样性不同,且相同植被下的细菌多样性随时间呈动态变化;可培养的菌落数和土壤微生物生物量之间并不存在显著的线性相关性;不同植被下菌落数与优势菌种类在95%的可信区间(P<0.05)相关性很好;不同植被下及同一植被下不同时间内优势菌形态、群落结构不同,所占百分比亦不同;对于部分优势菌的鉴定,农田和刺槐样地中可确定为粪产碱菌Alcaligenes faecalis和放射状根瘤菌Rhizobium radiobacter,其余植被下的鉴定到属.

关键词: 生态恢复, 土壤微生物多样性, BIOLOG鉴定系统 , 细菌

Abstract: The cultivable heterotrophic bacterial diversities under different vegetations were characterized by the traditional method from number, category and configuration aspects. Plate counts and some dominant bacterials were identified by BIOLOG microplate. The results show that the microbes under different vegetations have dissimilar diversities. The amounts and species of microbes changein different vegetation soil. In early spring, the variety of quantities and species of cultivable heterotrophic bacterial communities under different vegetation soil are not of prominent linear correlation, while becoming more and more prominent,with the climate changing. Between the cultivable microorganism and the soil microbial biomass significant linear correlation does not exist, while the CFU and species of preponderant microbes under different vegetations are of good relevance in the category of 95% credible interval (P<0.05). The percentages under different vegetations are different with different shapes and communities structures of the preponderant bacterias, even if in different time under the same vegetation. For some preponderant bacteria identification, Alcaligenes faecalis is found in farmland, Rhizobium radiobacter is found under Robinia pseudoacacia forest, and the remaining ones are identified to genus.

Key words: BIOLOG , bacteria, soil microbial diversity, ecorestoration

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