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J4 ›› 2009, Vol. 44 ›› Issue (5): 28-32.

• 论文 • 上一篇    下一篇

产广谱高效多烯类抗真菌抗生素的纺锤链霉菌SD07(Streptomyces netropsis)的分离及鉴定

金建玲, 王宠, 雷涛, 高培基   

  1. 山东大学生命科学学院 微生物技术国家重点实验室, 山东  济南 250100
  • 收稿日期:2008-10-27 出版日期:2009-05-16 发布日期:2009-11-18
  • 作者简介:金建玲(1965-),女,博士,主要研究方向为微生物遗传学、医学微生物学、微生物抗药性. Email:jinjianling@sdu.edu.cn
  • 基金资助:

    山东省自然科学基金资助项目(Y2008D15)

Isolation and classification of a Streptomyces netropsis strains SD07 which produces polyene macrolide antibiotics with
 broadspectrum  and high antifungal activity

JIN Jianling, WANG Chong, LEI Tao, GAO Peiji   

  1. State Key Laboratory Of Microbial Technology, School of Life Sciences, Shandong University, Jinan 250100, Shandong, China
  • Received:2008-10-27 Online:2009-05-16 Published:2009-11-18

摘要:

从山东省济南市南部山区土壤中分离得到1株具有广谱抗真菌活性的链霉菌SD07菌株。根据形态特征、生理生化反应和16S rDNA序列分析结果,链霉菌SD07为定为纺锤链霉菌(Streptomyces netropsis)。分离纯化了SD07?产生的抗真菌活性成分并获得其结晶CASD07。抗真菌活性检测结果发现: CASD07对酵母菌、担子菌、接合菌等多种真菌具有广谱抗菌作用。对CASD07进行性质研究发现,抗真菌活性成分CASD07抗菌谱广且抗菌活性高(约等于两倍的两性霉素B),是戊烯和七烯混合的多烯大环内酯类抗真菌抗生素。

关键词: 纺锤链霉菌;抗真菌抗生素;多烯大环内酯抗生素

Abstract:

Streptomyces sp. SD07 was isolated from the soil of the mountain area in the south of Jinan, Shandong province. This strain exhibited broadspectrum and high antifungal activity. Based on the results of preliminary morphological, cultural, physiological and biochemical studies on this strain, and its 16S rDNA sequences, strain SD07 was classified as Streptomyces netropsis. The antifungal compounds CASD07 were extracted from the mycelia and then crystallized,named as CASD07. CASD07 could inhibit many fungi such as Ascomycetes, Basidiomycetes, Zygomycetes and so on. The data from ultravioletvisible absorption and highperformance liquid chromatography / diode array detection (HPLC / DAD) indicated that CASD07 was a mixture of pentaene macrolide and heptaene macrolide antibiotics, and the major compounds were pentaene macrolide antibiotics. CASD07 has wider antifungal spectrum and higher antifungal activity(about two times Amphoteric B).

Key words: Streptomyces netropsis; antifungal antibiotics; pentaene macrolide antibiotics

中图分类号: 

  • R978.5
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