银杏叶提取物中总黄酮含量的分析方法研究

J4 ›› 2009, Vol. 44 ›› Issue (5): 40-44.

银杏叶提取物中总黄酮含量的分析方法研究

郭玉梅,杨景和*,吴霞

山东大学化学化工学院，山东济南 250100

收稿日期:2008-04-11 出版日期:2009-05-16 发布日期:2009-11-18
通讯作者: 杨景和(1941-),男,教授,博士生导师,研究方向为光分析和生物分析. Emial: yjh@sdu.edu.cn
作者简介:郭玉梅(1963-),女,高级实验师,硕士,研究方向为生物分析. Email: gym@sdu.edu.cn
基金资助:
国家自然科学基金资助项目(20575035);山东省自然科学基金资助项目(Y2003B02)

Study on the determination method of total flavonoids in ginkgo biloba extract

GUO Yumei, YANG Jinghe*, WU Xia

School of Chemistry and Chemical of Shandong University, Jinan 250100, China

Received:2008-04-11 Online:2009-05-16 Published:2009-11-18

摘要/Abstract

摘要：

提出了一种简便、灵敏地测定银杏叶提取物中总黄酮含量的新方法。实验结果表明银杏叶提取物经酸性条件下加热水解,使黄酮苷转化为苷元再与Al3+配位,所形成的配合物具有较强的荧光,可以槲皮素为标准品,采用荧光光度法测定其总黄酮含量。在选定条件下,槲皮素的浓度与荧光强度成线性关系,线性范围:0000?75～050?μg／mL, 检测限:045?g／mL(S／N=3),回归方程: If=7799?48+63210?86ρ×107(μg／mL),相关系数R=0999?9(P<0000?1),并用标准加入法进行回收实验和银杏叶提取物(GBE)中的总黄酮含量的测定,结果令人满意。

关键词: 荧光分光光度法;槲皮素;银杏叶提取物;总黄酮

Abstract:

A simple and sensitive new method for the determination of total flavonoids in ginkgo biloba extract was put forth. The experiment results indicated that the fluorescence intensity of ginkgo biloba extract (GBE) was greatly enhanced by heating in an acid medium and forming complex with Al3+. A fluorimetric method for determination of total flavonoids in GBE was proposed by taking quercetin as standard sample. Under the optimum conditions, a linear relationship was obtained between the fluorescence intensity and the concentration of quercetin in the range of 0000?75～050?μg／mL(R=0999?9; P<0000?1), and the detection limit was 045?ng／mL(S／N=3). This method was applied for determining the total flavonoids in GBE, the recovery test was put forth by standard addition method, and the satisfied results were obtained.

Key words: spectrofluorimetry; quercetin; ginkgo biloba extract; total flavonoids

中图分类号:

O657

. 银杏叶提取物中总黄酮含量的分析方法研究[J]. J4, 2009, 44(5): 40-44.

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