DOSY在寡糖混合物分析中的应用

J4 ›› 2013, Vol. 48 ›› Issue (1): 43-45.

DOSY在寡糖混合物分析中的应用

孙小婷1,靳岚2*

1.山东大学药学院微生物与生化药学研究所, 山东济南 250012;
2.山东大学国家糖工程技术研究中心, 山东济南 250012

收稿日期:2012-06-05 出版日期:2013-01-20 发布日期:2013-01-15
通讯作者: 靳岚(1981- ),女,副教授,博士,研究方向为寡糖结构生物学. Email: lanjin@sdu.edu.cn
作者简介:孙小婷(1988- ),女,硕士研究生,研究方向为微生物与生化药学. Email: sun110744@126.com
基金资助:
山东省自然科学基金资助项目(ZR2011CM038);山东大学自主创新基金资助项目(IIFSDU2010TS024)

Application of DOSY in oligosaccharide mixture analysis

SUN Xiao-ting1, JIN Lan2*

1. Institute of Biochemical and Biotechnological Drugs, School of Pharmaceutical Sciences,
Shandong University, Jinan 250012, Shandong, China;
2. National Glycoengineering Research Center, Shandong University, Jinan 250012, Shandong, China

Received:2012-06-05 Online:2013-01-20 Published:2013-01-15

摘要/Abstract

摘要：

糖链组成的高度多样化及组成单元本身的相似性,使得糖类化合物的分离纯化面临很大的困难,进而导致糖分子结构在很大程度上落后于蛋白质和核酸的结构研究。本论文将DOSY(diffusion ordered spectroscopy,扩散排序谱)应用于由D葡萄糖和β环糊精组成的混合物分析研究。结果表明该方法具有良好的结构分析效果,可以很好地研究混合物中单一组分的成分确定及结构表征,作为一种“NMR色谱”技术具有良好的应用前景。

关键词: 扩散排序谱;寡糖;混合物

Abstract:

Structures of carbohydrates are very much complex due to their multiple monosaccharide combinations and glycosidic linkages. Their isolation and purification are also difficult because of their structure similarity. Therefore, structure analysis of carbohydrate is limited and left far more behind that of proteins and nucleic acids. NMR (nuclear magnetic resonance) method of DOSY (diffusion ordered spectroscopy) has been used to analyze the mixture of D-glucose (D-Glc) and β-cyclodextrin (β-CD) in this paper. The results suggested that the so-called NMR chromatography can be successfully used as a routine and complement method in the analysis of oligosaccharide mixtures.

Key words: DOSY; oligosaccharide; mixture

孙小婷1,靳岚2*. DOSY在寡糖混合物分析中的应用[J]. J4, 2013, 48(1): 43-45.

SUN Xiao-ting1, JIN Lan2*. Application of DOSY in oligosaccharide mixture analysis[J]. J4, 2013, 48(1): 43-45.

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