束骨姜黄化学成分及其乙酰胆碱酯酶抑制活性

J4 ›› 2013, Vol. 48 ›› Issue (7): 20-23.

束骨姜黄化学成分及其乙酰胆碱酯酶抑制活性

张春梅1,王继达1,张永睿思1,范培红1,2*

1. 山东大学药学院, 山东济南 250012; 2. 陕西省植物化学重点实验室, 陕西宝鸡 721013

收稿日期:2013-04-25 发布日期:2013-12-03
通讯作者: 范培红(1979- ),女,副教授,研究方向为活性天然产物化学.Email:fanpeihong@sdu.edu.cn
作者简介:张春梅(1987- ),女,硕士研究生,研究方向为活性天然产物化学.Email:zcmxl2012@163.com
基金资助:
国家自然科学基金资助项目(81001616);山东省科学技术发展计划基金资助项目(2010GSF10223);山东省医药卫生科技发展计划基金资助项目(2011QZ012);陕西省重点实验室基金资助项目(12JS009)

Chemical constituents of Curcuma xanthorrhiza Roxb. and their acetylcholinesterase inhibitory activity

ZHANG Chun-mei1, WANG Ji-da1, ZHANG Yong-ruisi1, FAN Pei-hong1,2*

1.School of pharmaceutical sciences, Shandong University, Jinan 250012, Shandong, China
2. Shaanxi Key Laboratory of Phytochemistry, Baoji 721013, Shaanxi, China

Received:2013-04-25 Published:2013-12-03

摘要/Abstract

摘要：

采用硅胶层析、反相硅胶中压柱层析、高效液相等色谱技术对姜科姜黄属束骨姜黄(Curcuma xanthorrhiza Roxb.)根茎中的低极性化学成分进行了分离纯化,并通过MS和NMR等波谱技术确定了化合物结构,分离纯化化合物6个,分别鉴定为蓬莪术环氧酮(Ⅰ)、β谷甾醇(Ⅱ)、愈创木内酯Guai-1(10),3,5,7(11),8-pentaen-2-on-11,8-olide(Ⅲ)、郁金二酮(Ⅳ)、莪术双环烯酮(Ⅴ)、花侧柏烯(Ⅵ)。化合物Ⅲ、Ⅵ为首次从该科植物中分离得到,化合物Ⅳ、Ⅴ为首次从该种中分离得到。对所分离获得化合物进行了乙酰胆碱酯酶抑制活性测定,其中化合物Ⅳ、Ⅵ具有活性,化合物Ⅳ的TLC生物自显影最小抑制量为95μg。

关键词: 束骨姜黄;化学成分;乙酰胆碱酯酶抑制活性

Abstract:

Chemical constituents from Curcuma xanthorrhiza Roxb. (Zingiberaceae) rhizome were isolated and purified by column chromatography (silica gel, RPsilica gel MPLC, HPLC) and their structures were elucidated on the basis of spectral analyses. Six compounds were identified by MS and NMR spectroscopy as zederone(Ⅰ), βsitosterol(Ⅱ), Guai-1(10),3,5,7(11),8-pentaen-2-on-11,8-olide(Ⅲ), Curcumadione(Ⅳ), Curcumenone (Ⅴ), Cuparenol (Ⅵ). Compound Ⅲ and Ⅵ were isolated for the first time from the plants of Zingiberaceae, and compound Ⅳand Ⅴ were isolated from Curcuma xanthorrhiza Roxb. for the first time. Meanwhile, the acetylcholinesterase inhibitory activity of the isolated compounds was evaluated. Compounds Ⅳ and Ⅵ showed certain inhibitory activity, the minimum inhibitory quantity of Ⅳ on TLC bioautography test was 95μg.

Key words: Rhizoma Curcuma xanthorrhiza Roxb.; chemical constituent; acetylcholinesterase inhibitory activity

中图分类号:

R284.2

张春梅1,王继达1,张永睿思1,范培红1,2*. 束骨姜黄化学成分及其乙酰胆碱酯酶抑制活性[J]. J4, 2013, 48(7): 20-23.

ZHANG Chun-mei1, WANG Ji-da1, ZHANG Yong-ruisi1, FAN Pei-hong1,2*. Chemical constituents of Curcuma xanthorrhiza Roxb. and their acetylcholinesterase inhibitory activity[J]. J4, 2013, 48(7): 20-23.

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