红花性状标记杂交棉新品种鲁05H9 SSR#br#
指纹图谱构建及应用

doi:10.6040/j.issn.1671-9352.0.2013.286

山东大学学报（理学版） ›› 2014, Vol. 49 ›› Issue (1): 44-49.doi: 10.6040/j.issn.1671-9352.0.2013.286

红花性状标记杂交棉新品种鲁05H9 SSR#br# 指纹图谱构建及应用

赵军胜1,2，王秀丽1,2，高明伟1,2，王家宝1,2，王永翠1,2，#br# 陈莹1,2，姜辉1,2，杨静1,2，王留明1,2*

1.山东大学农学院， 2.山东棉花研究中心，山东济南 250100

收稿日期:2013-06-18 出版日期:2014-01-20 发布日期:2014-01-15
通讯作者: 王留明(1960- )，男，研究员，研究方向为棉花遗传育种. Email：scrcwlm1960@163.com
作者简介:赵军胜(1981- )，男，博士，副研究员，研究方向为棉花遗传育种. Email：zhaojs@saas.ac.cn
基金资助:
国家棉花产业技术体系资助项目(优质育种岗位CARS1810)；国家高技术研究发展计划资助项目(强优势棉花杂交种的创制与应用2011AA10A102)；山东省农业良种工程资助项目(2012LZ0503)

Construction and application of SSR fingerprints of hybrid #br# cotton variety Lu 05H9 with red-corolla marker trait

ZHAO Jun-sheng1,2, WANG Xiu-li1,2, GAO Ming-wei1,2, WANG Jia-bao1,2, WANG Yong-cui1,2, CHEN Ying1,2, JIANG Hui1,2, YANG Jing1,2, WANG Liu-ming1,2*

1. School of Agriculture Science, Shandong University, Jinan 250100, Shandong, China;
2. Shandong Cotton Research Center, Jinan 250100, Shandong, China

Received:2013-06-18 Online:2014-01-20 Published:2014-01-15

摘要/Abstract

摘要：

利用SSR分子标记技术对红花标记杂交棉新品种鲁05H9及其亲本材料进行多态性检测，从多态性高、重复性好的98对SSR核心引物中筛选出12对在双亲间具有多态性的引物、在F1中表现为杂合带的共显性标记。利用该12对引物构建了鲁05H9及其亲本的SSR指纹图谱。利用核心引物组合扩增的方法，可以鉴定鲁05H9的真实性和纯度，对于品种保护、纯度鉴定及杂交种推广具有极其重要的意义。

关键词: 杂交棉, 红花标记性状, SSR, 指纹图谱

Abstract:

SSR markers were used to analyze the fingerprinting of hybrid cotton variety Lu 05H9 with red-corolla marker trait. Lu 05H9 and its parents were amplified with 98 pairs of core primers with high polymorphisms and good repeatability. Out of them, 12 paris were detected to be polymorphic between the two parents, which were codominant markers since they amplified the different fragments between the two parents and heterozygosis bands in F1. Fingerprints of Lu05H9 were constructed with the amplification result of the 12 paris primers. It provides a method for seed genuineness and purity detection of hybrids. Due to its fast readability and large storage capacity, DNA fingerprinting of Lu05H9 is in favor of commercial applications.

Key words: fingerprinting, Hybrid cotton, SSR, Red-corolla marker trait

中图分类号:

S562.035

赵军胜1,2，王秀丽1,2，高明伟1,2，王家宝1,2，王永翠1,2，陈莹1,2，姜辉1,2，杨静1,2，王留明1,2*. 红花性状标记杂交棉新品种鲁05H9 SSR#br# 指纹图谱构建及应用[J]. 山东大学学报（理学版）, 2014, 49(1): 44-49.

ZHAO Jun-sheng1,2, WANG Xiu-li1,2, GAO Ming-wei1,2, WANG Jia-bao1,2, WANG Yong-cui1,2, CHEN Ying1,2, JIANG Hui1,2, YANG Jing1,2, WANG Liu-mi. Construction and application of SSR fingerprints of hybrid #br# cotton variety Lu 05H9 with red-corolla marker trait[J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2014, 49(1): 44-49.

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红花性状标记杂交棉新品种鲁05H9 SSR#br# 指纹图谱构建及应用

Construction and application of SSR fingerprints of hybrid #br# cotton variety Lu 05H9 with red-corolla marker trait

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