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山东大学学报（理学版） ›› 2015, Vol. 50 ›› Issue (01): 42-49.doi: 10.6040/j.issn.1671-9352.0.2014.286

• 论文 • 上一篇    下一篇

蒙古国西北部狼(Canis lupus)遗传多样性研究

刘元超1,2, 赵晶3, 初红军1,2,4, 董潭成4, 韩丽丽1,2, 陶永善2, 葛炎2   

  1. 1. 新疆大学资源与环境科学学院, 新疆 乌鲁木齐 830046;
    2. 新疆卡拉麦里山有蹄类自然保护区阿勒泰管理站, 新疆 阿勒泰 836500;
    3. 山东大学生命学院, 山东 济南 250100;
    4. 新疆农业大学动物科学学院, 新疆 乌鲁木齐 830052
  • 收稿日期:2014-06-23 修回日期:2015-01-04 出版日期:2015-01-20 发布日期:2015-01-24
  • 通讯作者: 初红军(1964-),男,高级工程师,博士生导师,研究方向为动物生态和保护生物学.E-mail:hongjunchu@vip.163.com E-mail:hongjunchu@vip.163.com
  • 作者简介:刘元超(1989-),男,硕士研究生,研究方向为动物生态和保护生物学.E-mail:420741463@qq.com
  • 基金资助:
    新疆自治区维吾尔科技支撑计划资助项目(201233131);新疆维吾尔自治区科技支疆资助项目(201291148);卡拉麦里山有蹄类自然保护区阿勒泰管理站中央财政森林生态效益补偿基金资助项目;科技部科技基础性工作专项重大资助项目(2013FY110300)

Genetic diversities of wolves mtDNA D-loop in northwestern Mongolian

LIU Yuan-chao1,2, ZHAO Jing3, CHU Hong-jun1,2,4, DONG Tan-cheng4, HAN Li-li1,2, TAO Yong-shan2, GE Yan2   

  1. 1. College of Resources and Environment Sciences, Xinjiang University, Urumqi 830046, Xinjiang, China;
    2. Altay Management Station, Mt. Kalamaili Ungulate Nature Reserve, Altay 836500, Xinjiang, China;
    3. College of Biology Sciences, Shandong University, Jinan 250100, Shandong, China;
    4. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
  • Received:2014-06-23 Revised:2015-01-04 Online:2015-01-20 Published:2015-01-24

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摘要： 采用PCR和测序技术,分析了30只蒙古国西北部狼线粒体DNA(mtDNA)D-loop区519 bp核酸序列,基于 mtDNA D-loop区序列对蒙古国狼和NCBI公布的其它狼种群进行系统树构建。结果显示:蒙古国狼存在7种单倍型,检测到29处变异位点,占所分析位点总数的4.9%,各单倍型间的遗传距离为0.006～0.024平均遗传距离是0.004±0.001。单倍型多样性为0.952±0.096,核苷酸多性为0.011 60±0.006 46。聚类分析表明,蒙古国狼分布在2个支系中,与中国新疆、内蒙古种群亲缘关系较近,与中国青海、西藏、喜马拉雅山脉种群的亲缘关系较远。蒙古国西北部狼种群具有比较丰富的遗传多样性,在进化的历史过程中受到其他狼种群的影响较大。中国青海、西藏、喜马拉雅山附近的狼种群应同属于一个群体,该研究为蒙古国乃其与我国毗邻区域狼的遗传多样性保护提供了理论依据。

关键词: mtDNA D-loop, 狼, 母系起源, 遗传多样性

Abstract: Genetic diversities of mitochondrial DNA (mtDNA) D-loop and maternal origin of northwestern Mongolian Canis lupus were investigated. Thirty 519 bp mtDNA D-loop sequences were analyzed by PCR and sequencing techniques, and the phylogenetic trees were constructed based on the mtDNA D-loop sequences of northwestern Mongolian Canis lupus samples and other wolves D-loop sequence available from NCBI. There were 7 unique haplotypes with 29 polymorphic sites in northwestern Mongolian Canis lupus, which account for 4.9% of 519 bp sequence analyzed. The haplotype diversity(h) and nucleotide diversity(π) were 0.952±0.096 and 0.011 60±0.006 46, respectively. Moreover, northwestern Mongolian Canis lupus distributed in two branches, and are closely related to Xinjiang of China, Inner Mongolia of China, whereas they are less related to Qinghai of China, Tibet of China, and the Himalayan population. The northwest Mongolia Canis lupus have rather abundant genetic diversity and were greatly affected by other Canis lupus species in history. Qinghai of China, Tibet of China, and the Himalayan Canis lupus should belong to a group.

Key words: Canis lupus, genetic diversity, mtDNA D-loop, maternal origin

中图分类号: 

  • Q38
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[1] 杜聪聪,赵晶,初红军,刘元超,端肖楠,陈刚. 新疆河狸mtDNA D-loop HV-Ⅰ区的遗传多样性研究[J]. 山东大学学报（理学版）, 2016, 51(3): 19-28.
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