功能化多壁碳纳米管对NHFB细胞的毒性研究

J4 ›› 2010, Vol. 45 ›› Issue (5): 6-11.

功能化多壁碳纳米管对NHFB细胞的毒性研究

李秀娥¹,高素莲²,张秋³*

1. 山东大学药学院, 山东济南 250012; 2. 山东大学环境研究院,山东济南 250100;
3. 山东大学化学与化工学院, 山东济南 250100

收稿日期:2009-10-19 出版日期:2010-05-16 发布日期:2010-05-24
通讯作者: 张秋(1976-), 女,博士,讲师,主要从事药物及纳米材料毒理学研究.
作者简介:李秀娥(1984-),女,硕士研究生,主要从事药物及纳米材料毒理学研究. Email: lixiue0567@163.com
基金资助:
国家重大基础研究发展计划(973)资助项目(2010CB933504)

Study of the toxicity of functionalized multi-walled carbon nanotubes to NHFB cells

LI Xiu-e¹, GAO Shu-lian², ZHANG Qiu^3*

1. School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, China;
2. School of Environmental Science and Engineering, Shandong University, Jinan 250100, Shandong, China;
3. School of Chemical Science and Engineering, Jinan 250100, Shandong, China

Received:2009-10-19 Online:2010-05-16 Published:2010-05-24

摘要/Abstract

摘要：

以人的正常上皮成纤维细胞(normal human fibroblasts, NHFB)为模型, 应用WST-1方法对本课题组前期合成的功能化多壁碳纳米管(functionalized multiwalled carbonnanotubes, f-MWNTs)库进行细胞毒性筛选;选取细胞毒性差异较大的几种f-WNTs,研究细胞对其摄入情况及其对细胞增殖和活性氧(reactive oxygen species, ROS)产生的影响规律。结果表明:经过不同表面修饰的多壁碳纳米管具有不同的细胞毒性; f-MWNTs能够进入细胞,且其对细胞增殖及ROS的产生均呈显著的剂量依赖关系。

关键词: 功能化多壁碳纳米管;细胞毒性;氧化应激

Abstract:

The cytotoxicity of the functionalized multi-walled carbon nanotubes (f-MWCNTs) library, previously synthesized by our group, to normal human fibroblasts (NHFB) was investigated by WST-1 assay. The effects of selected f-MWCNTs with different cytotoxicity on cellular uptake and proliferation, and oxidative stress in NHFB were studied. It was found that different surface modification of MWCNTs could lead to different cytotoxicity, and f-MWCNTs could be uptaken by NHFB and induce cytotoxity and reactive oxygen species in NHFB in a dose dependent manner.

Key words: functionalized multi-walled carbon nanotubes; cytotoxicity; oxidative stress

李秀娥1,高素莲2,张秋3*. 功能化多壁碳纳米管对NHFB细胞的毒性研究[J]. J4, 2010, 45(5): 6-11.

LI Xiu-e1, GAO Shu-lian2, ZHANG Qiu3*. Study of the toxicity of functionalized multi-walled carbon nanotubes to NHFB cells[J]. J4, 2010, 45(5): 6-11.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed