光谱法研究nC60纳米颗粒与牛血红蛋白的相互作用

doi:10.6040/j.issn.1671-9352.0.2014.330

山东大学学报（理学版） ›› 2015, Vol. 50 ›› Issue (01): 56-61.doi: 10.6040/j.issn.1671-9352.0.2014.330

光谱法研究nC₆₀纳米颗粒与牛血红蛋白的相互作用

姚尧, 林南宇, 刘淑芳

山东大学公共卫生学院, 山东济南 250012

收稿日期:2014-07-10 修回日期:2014-10-29 出版日期:2015-01-20 发布日期:2015-01-24
通讯作者: 刘淑芳(1979-),女,博士,研究方向为纳米材料的生物学效应.E-mail:liushufang@sdu.edu.cn E-mail:liushufang@sdu.edu.cn
作者简介:姚尧(1989-),男,硕士研究生,研究方向为纳米材料的生物学效应.E-mail:yaoysdu@gmail.com
基金资助:
山东省博士后创新项目专项资金资助项目(200902015)

Spectroscopic study on the interaction between nC₆₀ nanoparticles and bovine hemoglobin

YAO Yao, LIN Nan-yu, LIU Shu-fang

School of Public Health, Shandong University, Jinan 250012, Shandong, China

Received:2014-07-10 Revised:2014-10-29 Online:2015-01-20 Published:2015-01-24

摘要/Abstract

摘要： 用几种光谱方法研究了nC₆₀纳米颗粒与牛血红蛋白之间的相互作用。用溶剂置换法制备nC₆₀的水分散液,并用紫外-可见分光光度法、动态光散射和透射电子显微镜技术对nC₆₀纳米颗粒进行表征;用紫外-可见分光光谱、荧光光谱、同步荧光光谱研究nC₆₀与牛血红蛋白之间的作用。结果表明,nC₆₀纳米颗粒对牛血红蛋白的紫外-可见吸收光谱有一定程度的改变作用;nC₆₀可猝灭牛血红蛋白的内源荧光,且最大发射波长发生明显的蓝移现象;随着nC₆₀浓度的增加,酪氨酸残基和色氨酸残基的同步荧光强度降低,其中酪氨酸残基峰位发生蓝移,而色氨酸残基峰位基本保持不变。研究表明nC₆₀与牛血红蛋白之间存在一定的相互作用;nC₆₀可引起牛血红蛋白构象的改变,酪氨酸残基所处微环境的极性减小,nC₆₀与牛血红蛋白的作用位置更接近酪氨酸残基。

关键词: 富勒烯, 荧光, 牛血红蛋白, C₆₀

Abstract: Several spectroscopic methods were used to study the interaction between nC₆₀ nanoparticles and bovine hemoglobin. The nC₆₀ nanoparticles dispersion in water was prepared by solvent replacement method and characterized using UV-Vis spectrometry, dynamic light scattering and transmission electron microscopy. The interaction between nC₆₀ and bovine hemoglobin was investigated by UV-Vis spectrometry, fluorescence spectroscopy and synchronous fluorescence spectroscopy. The result indicated that the UV-Visible absorption spectrum of bovine hemoglobin was changed to some extent by nC₆₀ nanoparticles. The intrinsic fluorescence of bovine hemoglobin could be quenched by nC₆₀ and the maximum emission wavelength showed obvious blue-shift. With the increasing concentrations of nC₆₀, the synchronous fluorescence intensity of tyrosine and tryptophan residues decreased gradually, and the fluorescence peak of tyrosine residues exhibited a blue shift while the fluorescence peak of tryptophan residues remained unchanged. The research showed that the binding of nC₆₀to bovine hemoglobin could cause the conformation change of bovine hemoglobin. The microenvironment polarity around tyrosine residues decreased, indicating the binding site of nC₆₀ to bovine hemoglobin was located near tyrosine residues.

Key words: fullerene, fluorescence, C₆₀, bovine hemoglobin

中图分类号:

O657.3

姚尧, 林南宇, 刘淑芳. 光谱法研究nC₆₀纳米颗粒与牛血红蛋白的相互作用[J]. 山东大学学报（理学版）, 2015, 50(01): 56-61.

YAO Yao, LIN Nan-yu, LIU Shu-fang. Spectroscopic study on the interaction between nC₆₀ nanoparticles and bovine hemoglobin[J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2015, 50(01): 56-61.

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光谱法研究nC₆₀纳米颗粒与牛血红蛋白的相互作用

Spectroscopic study on the interaction between nC₆₀ nanoparticles and bovine hemoglobin

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