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山东大学学报（理学版） ›› 2016, Vol. 51 ›› Issue (3): 29-33.doi: 10.6040/j.issn.1671-9352.0.2015.456

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荧光光谱法研究羟基化单壁碳纳米管与牛血清白蛋白/血红蛋白的相互作用

刘玉梅,王海蓉,刘淑芳*   

  1. 山东大学公共卫生学院, 山东 济南 250012
  • 收稿日期:2015-09-23 出版日期:2016-03-20 发布日期:2016-04-07
  • 通讯作者: 刘淑芳(1979— ),女,博士,研究方向为纳米材料的生物学效应. E-mail:liushufang@sdu.edu.cn E-mail:15063355169@163.com
  • 作者简介:刘玉梅(1989— ),女,硕士研究生,研究方向为纳米材料的生物学效应.E-mail:15063355169@163.com
  • 基金资助:
    中国博士后科学基金面上资助项目(20090461214)

Fluorescence spectrometry study on the interaction between hydroxyl modified single-wall carbon nanotubes and bovine serum albumin/hemoglobin

LIU Yu-mei, WANG Hai-rong, LIU Shu-fang*   

  1. School of Public Health, Shandong University, Jinan 250012, Shandong, China
  • Received:2015-09-23 Online:2016-03-20 Published:2016-04-07

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摘要： 纳米材料与蛋白质等生物大分子的相互作用是纳米材料生物安全性的重要内容。为了解羟基化单壁碳纳米管与牛血清白蛋白/血红蛋白的相互作用特点,选择两种不同长度的羟基化单壁碳纳米管(Short-SWNTs-OH、Long-SWNTs-OH),利用荧光光谱法和同步荧光光谱法分别分析在生理条件下它们与牛血清白蛋白/血红蛋白的相互作用。荧光光谱显示,两种羟基化单壁碳纳米管可以猝灭牛血清白蛋白和牛血红蛋白的内源荧光,且猝灭效应随碳纳米管的浓度增大而增强, 对牛血清白蛋白,Short-SWNTs-OH的荧光猝灭作用强于Long-SWNTs-OH;Short-SWNTs-OH和Long-SWNTs-OH对牛血红蛋白的荧光猝灭作用相差不大;同步荧光光谱显示,Short-SWNTs-OH对牛血清白蛋白的色氨酸残基(Trp)荧光猝灭作用强于酪氨酸残基(Tyr)。实验结果表明,碳纳米管的形貌特征及蛋白质的类型对碳纳米管与蛋白质的相互作用存在一定的影响:与牛血红蛋白相比,羟基化单壁碳纳米管更易于和牛血清白蛋白结合,羟基化单壁碳纳米管的长度、蛋白质的类型对它们之间的相互作用存在一定的影响,两种碳纳米管与牛血清白蛋白的作用位置更接近色氨酸残基。该工作可为全面评价包括碳纳米管在内的纳米材料的生物安全性提供参考。

关键词: 碳纳米管, 牛血红蛋白, 牛血清白蛋白, 荧光光谱

Abstract: The interaction between nanometer materials and biological macromolecules such as protein is an important content of biological safety of nano materials. This experiment aimed to study the interaction between hydroxyl modified single-wall carbon nanotubes and bovine serum albumin/hemoglobin. Two hydroxyl modified single-walled carbon nanotubes with different lengths(Short-SWNTs-OH and Long-SWNTs-OH)were chosen. The interaction between hydroxyl modified single-wall carbon nanotubes and bovine serum albumin/hemoglobin were investigated by fluorescence spectroscopy and synchronous fluorescence spectrometry under physiological conditions. The fluorescence spectra showed the intrinsic fluorescence of bovine serum albumin/hemoglobin could be quenched by two hydroxyl modified single-wall carbon nanotubes with concentration-effect relationship. For bovine serum albumin, the fluorescence quenching effect of Short-SWNTs-OH was more than that of Long-SWNTs-OH, while less different quenching effect of two carbon nanotubes was observed for bovine hemoglobin. The synchronous fluorescence spectra showed that the fluorescence quenching effect caused by Short-SWNTs-OH on tryptophan(Trp)residues was stronger than that of tyrosine(Tyr)residues. The above experimental results indicate that the carbon nanotubes morphology and protein type could 山 东 大 学 学 报 (理 学 版)第51卷 - 第3期刘玉梅,等:荧光光谱法研究羟基化单壁碳纳米管与牛血清白蛋白/血红蛋白的相互作用 \=-influence the interaction between them: hydroxyl single-walled carbon nanotubes combine more easily with bovine serum albumin compared to bovine hemoglobin, the length of hydroxyl single-walled carbon nanotubes and the types of protein could influence the interaction between carbon nanotubes and protein and the binding site of two carbon nanotubes to bovine serum albumin was located near tryptophan(Trp)residues. This study may provide reference for the biological safety evaluation of nanomaterials including carbon nanotubes.

Key words: carbon nanotubes, bovine serum albumin, bovine hemoglobin, fluorescence spectroscopy

中图分类号: 

  • O657.3
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