正交试验优选核桃楸皮中
有效成分的提取工艺

J4 ›› 2013, Vol. 48 ›› Issue (11): 23-26.

正交试验优选核桃楸皮中
有效成分的提取工艺

方薇1,王一男2,蔡小燕1,曹治家1,陈洪升1,张咏莉1*

1. 广东药学院基础学院, 广东广州 510006; 2. 沈阳药科大学药学院, 辽宁沈阳 110016

收稿日期:2013-04-19 出版日期:2013-11-20 发布日期:2013-11-25
通讯作者: 张咏莉(1973- ),女,副教授,硕士生导师,研究方向为中药材抗肿瘤药效及分子机制研究.Email: zyl5198@163.com
作者简介:方薇(1989- ),女,硕士研究生,研究方向为中药材抗肿瘤药效及分子机制研究.Email:Fangwei635@163.com
基金资助:
国家自然科学基金青年资助项目(81102753);中山市科技局资助项目(2009H021)

Optimization the extraction process of effective compositions
from Cortex Juglandis Mandshuricae

FANG Wei1, WANG Yi-nan2, CAI Xiao-yan1, CAO Zhi-jia1, CHEN Hong-sheng1, ZHANG Yong-li1*

1. School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong, China;
2. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, Liaoning, China

Received:2013-04-19 Online:2013-11-20 Published:2013-11-25

摘要/Abstract

摘要：

对核桃楸树皮粗粉进行提取分离,鉴定所得产物并优化提取工艺。将核桃楸药材粗粉,经乙醇回流提取,乙酸乙酯和氯仿萃取,后经反复硅胶柱层析梯度洗脱得到3种黄酮类化合物。以正交试验考查最佳提取工艺。通过正交试验得到提取核桃楸树皮有效成分的最佳工艺为以8倍核桃楸粗粉质量的75％乙醇回流提取3次,每次历时15h,并用核磁共振的方法对所得化合物进行鉴定。乙醇回流提取并分离得到核桃楸树皮中的杨梅素、柚皮素、橙皮素3种黄酮类单体是可行的。

关键词: 核桃楸;提取分离;正交试验

Abstract:

To optimize the extraction process of effective compositions from Cortex Juglandis Mandshuricae. Cortex Juglandis Mandshuricae were extracted with ethanol,concentrated with ethyl acetate and chloroform and eluted gradiently on silica gel to get 3 kinds of flavonoids compounds. Orthogonal experiments were used to examine the best extraction process. The best extraction process is to use 75％ ethanol with 8 times the weight of Cortex Juglandis Mandshuricae scoured grist to reflux extract 3 times with 1.5h each time.The products identified by nuclear magnetic resonance methods. Myricetin, naringenin and hesperitin are the main contents of the Cortex Juglandis Mandshuricae, and the ethanol refluxing extraction process is feasible.

Key words: Juglandis Mandshuricae; extraction and separation; orthogonal experiment

中图分类号:

R914.4

方薇1,王一男2,蔡小燕1,曹治家1,陈洪升1,张咏莉1*. 正交试验优选核桃楸皮中
有效成分的提取工艺[J]. J4, 2013, 48(11): 23-26.

FANG Wei1, WANG Yi-nan2, CAI Xiao-yan1, CAO Zhi-jia1, CHEN Hong-sheng1, ZHANG Yong-li1*. Optimization the extraction process of effective compositions
from Cortex Juglandis Mandshuricae[J]. J4, 2013, 48(11): 23-26.

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