关键词: 杂交F1代, 同工酶, 皱纹盘鲍, 黑足鲍

Abstract: The vertical polyacrylamide gel electrophoresis was used to compare the expressions patterns of seven isozymes (SOD,MDH,POD,EST,CAT,ODH and LDH) in both viscera and muscles of Haliotis discus hannai， Haliotis iris and their F1 hybrids,in order to probe how their parents’ genetic affect the expression of the Hybrids’gene in terms of isozymes,and this may provide the molecular basis of the difference among the three strains of abalone.The results shows that there were similar isozyme phenotypes such as ODH in both tissues as well as POD and EST in the muscles of the parents. While there did exist difference in isozyme zymography between the parents, for example MDH, LDH and EST in the viscera.The hybrids were more similar with Haliotis discus hannai, while have larger difference with Haliotis iris, the isozyme zymography of POD, EST, LDH, SOD and MDH in the muscles of F1 hybrids and mother abalone were all the same. Thus, the gene expression of isozymes in the first generation hybrids mainly succeed their mothers.

Key words: Haliotis discus hannai, isozymes, Haliotis iris, F1 hybridization