盐诱导乳清蛋白冷凝胶对双歧杆菌的保护作用

盐诱导乳清蛋白冷凝胶对双歧杆菌的保护作用

张久龙，孟祥晨*

东北农业大学乳品科学教育部重点实验室，黑龙江哈尔滨 150030

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-10-24 发布日期:2006-10-24
通讯作者: 孟祥晨

Protection of salt-induced whey protein cold-set gels on Bifidobacterium sp.

ZHANG Jiu-long, MENG Xiang-chen*

Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, Heilongjiang, China

Received:1900-01-01 Revised:1900-01-01 Online:2006-10-24 Published:2006-10-24
Contact: MENG Xiang-chen

摘要/Abstract

摘要：

探讨盐诱导乳清分离蛋白（whey protein isolates, WPI）形成的包含双歧杆菌的乳清蛋白冷凝胶（cold-set gels of whey protein containing bifidobacteria，CGWPCB）对双歧杆菌的保护作用。借助扫描电子显微镜和物性仪观察凝胶结构，评价以氯化钙为凝胶剂，形成的CGWPCB对双歧杆菌的保护作用。结果表明，7.5mmol/L CaCl2诱导8% WPI形成的网络状冷凝在pH 1.5的酸环境下对双歧杆菌的保护性最好，作用120min后菌落数下降3个数量级，菌体存活率达到0.42%。说明制备的乳清蛋白冷凝胶对双歧杆菌有良好的保护性，为益生菌产品的开发和应用提供了参考。

关键词: 乳清蛋白, 保护作用, 双歧杆菌, 盐诱导, 冷凝胶

Abstract:

The protection of salt-induced whey protein isolates (WPI) on Bifidobacterium was investigated. The protection on Bifidobacterium was determined by experiments of acid tolerance, and the gel structure was observed by scanning electron microscope and texture analyser. The results show that the network cold-set gels of 8% WPI solution induced by 7.5mmol/L CaCl2 can give the best protection on bifidobactetrial cells with an acid condition of pH 1.5. The number of bifidobacterial cells decreases by three order of magnitude, and the survival rate of bifidobacteria is 0.42% by treatment in the acid condition of pH 1.5 for 120 minutes, which suggests better protection on bifodobacterial cells by cold-set gels of WPI can be achieved, and can provide a strategy in the development and application of probiotics.

Key words: protection

, Bifidobacterium sp., salt-induction, cold-set gels,

whey protein

中图分类号:

Q939.9

张久龙,孟祥晨* . 盐诱导乳清蛋白冷凝胶对双歧杆菌的保护作用[J]. J4, 2008, 43(7): 65-68 .

ZHANG Jiu-long,MENG Xiang-chen* .

Protection of salt-induced whey protein cold-set gels on Bifidobacterium sp.

[J]. J4, 2008, 43(7): 65-68 .

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