JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2016, Vol. 51 ›› Issue (7): 121-125.doi: 10.6040/j.issn.1671-9352.0.2016.119

Previous Articles     Next Articles

A novel small peptide ligand for detection of Alzheimer-associated neuronal thread protein

ZHANG Yan1,2, WEI Yu-ping1, ZHANG Liang1,2, FU Yan-kai1,2, XU Jian-dong1, XU Xia1*   

  1. 1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2016-03-23 Online:2016-07-20 Published:2016-07-27

PDF (PC)

850

Abstract: Alzheimer-associated neuronal thread protein(AD7c-NTP)as a sensitive, easily obtained and noninvasive biomarker for AD disease detection. This research is aim to detect AD7c-NTP protein. A novel peptide for detecting AD7c-NTP was developed using molecular simulations and the peptide was synthesized. The recombinant protein was expressed. The bound protein was tested using chromatography and the mimic urine was detected as well. The peptide of DEWH is able to bind AD7c-NTP with the adsorption rate of around 85% and the linearity range is 200-900 μg/L. This novel peptide could provide an easy and repeatable method for detecting AD disease, which is critical for early diagnosis and postponing disease progression with proper treatment.

Key words: Alzheimer disease, AD7c-NTP, Affinity peptide

CLC Number: 

  • O629.72
[1] Alzheimers Association. 2015 Alzheimers disease facts and figures[J]. Alzheimers & Dementia, 2015, 11(3):332-384.
[2] SORENSEN A A. Alzheimers disease research: scientific productivity and impact of the top 100 investigators in the field[J]. Journal of Alzheimers Disease, 2009, 16(3):451-465.
[3] LUKENS J N, VAN DEERLIN V, CLARK C M, et al. Comparisons of telomere lengths in peripheral blood and cerebellum in Alzheimers disease[J]. Alzheimers & Dementia, 2009, 5(6):463-469.
[4] MONTE S M, WANDS J R. The AD7c-NTP neuronal thread protein biomarker for detecting Alzheimers disease[J]. Journal of Alzheimers Disease, 2001, 3(3):345-353.
[5] WANG C, CUI Y, YANG J, et al. Combining serum and urine biomarkers in the early diagnosis of mild cognitive impairment that evolves into Alzheimers disease in patients with the apolipoprotein E ε4 genotype[J]. Biomarkers, 2015, 20(1):84-88.
[6] GOODMAN I, GOLDEN G, FLITMAN S, et al. A multi-center blinded prospective study of urine neural thread protein measurements in patients with suspected Alzheimers disease[J]. Journal of the American Medical Directors Association, 2007, 8(1):21-30.
[7] YOUN Y C, PARK K W, HAN S H, et al. Urine neural thread protein measurements in Alzheimer disease[J]. Journal of the American Medical Directors Association, 2011, 12(5):372-376.
[8] LEVY S, MCCONVILLE M, LAZARO G A, et al. Competitive ELISA studies of neural thread protein in urine in Alzheimers disease[J]. Journal of Clinical Laboratory Analysis, 2007, 21(1):24-33.
[9] LIPMAN N S, JACKSON L R, TRUDEL L J, et al. Monoclonal versus polyclonal antibodies: distinguishing characteristics, applications, and information resources[J]. ILAR Journal, 2005, 46(3):258-268.
[10] YANG J, YAN R, ROY A, et al. The I-TASSER Suite: protein structure and function prediction[J]. Nature Methods, 2015, 12(1):7-8.
[11] ROY A, KUCUKURAL A, ZHANG Y. I-TASSER: a unified platform for automated protein structure and function prediction[J]. Nature Protocols, 2010, 5(4):725-738.
[12] ZHANG Y. I-TASSER server for protein 3D structure prediction[J]. BMC bioinformatics, 2008, 9(1):40-47.
[13] MONTE S M, GHANBARI K, FREY W H, et al. Characterization of the AD7C-NTP cDNA expression in Alzheimers disease and measurement of a 41-kD protein in cerebrospinal fluid[J]. Journal of Clinical Investigation, 1997, 100(12):3093-3104.
[14] WEI Y P, XU J D, ZHANG L, et al. Development of novel small peptide ligands for antibody purification[J]. RSC Advances, 2015, 5(82):67093-67096.
[15] ZHANG J, SHI S L. A literature review of AD7c-ntp as a biomarker for Alzheimers disease[J]. Annals of Indian Academy Neurology, 2013, 16(3):307-319.
[16] MA L, CHEN J, WANG R, et al. The level of Alzheimer-associated neuronal thread protein in urine may be an important biomarker of mild cognitive impairment[J]. Journal of Clinical Neuroscience, 2015, 22(4):649-652.
No related articles found!
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE)
Supported by Beijing Magtech Co.Ltd