JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2016, Vol. 51 ›› Issue (7): 121-125.doi: 10.6040/j.issn.1671-9352.0.2016.119

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A novel small peptide ligand for detection of Alzheimer-associated neuronal thread protein

ZHANG Yan1,2, WEI Yu-ping1, ZHANG Liang1,2, FU Yan-kai1,2, XU Jian-dong1, XU Xia1*   

  1. 1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2016-03-23 Online:2016-07-20 Published:2016-07-27

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Abstract: Alzheimer-associated neuronal thread protein(AD7c-NTP)as a sensitive, easily obtained and noninvasive biomarker for AD disease detection. This research is aim to detect AD7c-NTP protein. A novel peptide for detecting AD7c-NTP was developed using molecular simulations and the peptide was synthesized. The recombinant protein was expressed. The bound protein was tested using chromatography and the mimic urine was detected as well. The peptide of DEWH is able to bind AD7c-NTP with the adsorption rate of around 85% and the linearity range is 200-900 μg/L. This novel peptide could provide an easy and repeatable method for detecting AD disease, which is critical for early diagnosis and postponing disease progression with proper treatment.

Key words: Alzheimer disease, AD7c-NTP, Affinity peptide

CLC Number: 

  • O629.72
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