Abstract:

 To optimize the extraction process of effective compositions from Cortex Juglandis Mandshuricae. Cortex Juglandis Mandshuricae were extracted with ethanol,concentrated with ethyl acetate and chloroform and eluted gradiently on silica gel to get 3 kinds of flavonoids compounds. Orthogonal experiments were used to examine the best extraction process.  The best extraction process is to use 75％ ethanol with 8 times the weight of Cortex Juglandis Mandshuricae scoured grist to reflux extract 3 times with 1.5h each time.The products identified by nuclear magnetic resonance methods. Myricetin, naringenin and hesperitin are the main contents of the Cortex Juglandis Mandshuricae, and the ethanol refluxing extraction process is feasible.

Key words: Juglandis Mandshuricae; extraction and separation; orthogonal experiment