Abstract:

Existing methods for analyzing PCBs in animal muscle require multiple and laborious steps prior to quantitation. In this paper, a method for determination of Polychlorinated Biphenyls(PCBs) in tuna by Stable Isotope Dilution Gas Chromatography-Mass Spectrometry(GCMS) has been proposed. Uncertainty source of the method has been analyzed systematically. By analyzing of standard material PCB28, the uncertainty factors of testing process were evaluated and synthesized. When the result for the determination of PCB28 was 17.7ng／g, the expanded uncertainty was 1.9ng／g, k=2, p=95％. This paper evaluated comprehensively the predominant influences on the uncertainty, some methods used to improve the quality control were also discussed.

Key words: Gas Chromatography-Mass Spectrometry (GCMS); tuna; polychlorinated biphenyls(PCBs); uncertainty