JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2024, Vol. 59 ›› Issue (1): 139-150.doi: 10.6040/j.issn.1671-9352.0.2023.116

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Growth and physiological mechanism study of H. crustuliniforme and Lonicera japonica symbiotic relationship under salt tolerance

Deyu MU1(),Nan LI1,Tao WANG2,*(),Jin LIU1,Zongzhao MU3,Jingchuan ZHANG4   

  1. 1. Institute of Ornamental Plants, Shandong Jianzhu University, Jinan 251010, Shandong, China
    2. Weihai City Management Comprehensive Service Center, Weihai 264200, Shandong, China
    3. Shandong Forestry Foreign Investment and Engineering Project Management Station, Jinan 251014, Shandong, China
    4. Forestry Industry Development Center of Rushan Natural Resources Bureau, Weihai 264200, Shandong, China
  • Received:2023-03-23 Online:2024-01-20 Published:2024-01-19
  • Contact: Tao WANG E-mail:mudeyu@yeah.net;whaiwangtao@163.com

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Abstract:

Lonicera japonica and Hebeloma crustuliniforme were used as materials in this study. 0, 60, 90 and 120 mmol/L NaCl salt treatments were taken out to study the rule of H. crustuliniforme colonization in plant roots and analyze the mechanism of H. crustuliniforme on the growth and physiological characteristics of L. japonica seedlings. The results showed that with the increase in salt concentration, the proline content in the inoculated group plants was 35.98%, 141.28%, 71.66% and 8.79% higher than that of the control group, respectively. Under the treatment of 90 and 120 mml/L salt concentration, the salt tolerance of L.japonica plants was improved by the H. crustuliniforme. The results of correlation and two-way ANOVA indicated thatnew shoot growth and the number of branch growth were positively correlated with the biomass, and proline showed a highly significant negative correlation with the number of branch growth. H. crustuliniforme could significantly reduce the host seedling height growth and increase the host proline content significantly. The inoculation with H. crustuliniforme treatment could increase the proline accumulation of L. japonica seedlings to resist salt adversity under salt stress.

Key words: Hebeloma crustuliniforme, Lonicera japonica, mycorrhizal fungi, physiological characteristics, salt resistance

CLC Number: 

  • S793.9

Table 1

Experimental design"

浓度/(mmol·L-1) 对照组/株 接菌组/株 合计/株
0 6 6 12
60 6 6 12
90 6 6 12
120 6 6 12
合计 24 24 48

Fig.1

Hebeloma crustuliniforme colonizes in the root system of Lonicera japonica"

Table 2

Intensity of colonizationby Hebeloma crustuliniforme on Lonicera japonica cuttings"

编号 处理浓度/(mmol/L) 侵染率平均值/%
1 0 66.7±7.2
2 60 70.0±4.7
3 90 66.7±2.7
4 120 63.3±7.2

Fig.2

Dry weight and fresh weight of Lonicera japonica Note: By Duncan's multiple comparisons, different upper or lower case letters on the means of each indicator indicate significant differences (P < 0.05)."

Fig.3

Hight growth of seedlings in each group after salt stress treatment"

Fig.4

Growth of Lonicera japonica new shoots after salt stress treatment"

Fig.5

Branch growth of Lonicera japonica after salt stress treatment"

Table 3

Two-way ANOVA analysis of the response of Hebeloma crustuliniformeto growth indexes of Lonicera japonica under salt stress"

处理 苗高增长量 新梢生长量 分枝增长数 总鲜质量 总干质量 地下鲜质量 地上鲜质量 地下干质量 地上干重
接菌 10.302* 381.306 3.025 0.091 0.482 0.441 0.132 0.027 0.279
盐胁迫 2.772 23.156 4.292 18.534* 12.004* 2.783 8.809* 0.084 10.322*
接菌+盐胁迫 2.336 456.740* 5.292 3.666 6.385 0.701 7.005* 0.008 6.687

Fig.6

Leaf chlorophyll concentrations of Lonicera japonica"

Fig.7

Leaf relative water content of Lonicera japonica"

Fig.8

Leaf proline content of Lonicera japonica"

Fig.9

Leaf soluble protein content of Lonicera japonica"

Table 4

Two-way ANOVA analysis of the response of Hebeloma crustuliniforme to physiological indexes of Lonicera japonica under NaCl salt stress"

处理 叶绿素a 叶绿素b 叶绿素 相对含水量 脯氨酸含量 可溶性蛋白含量
接菌 0.053 0.000 0.063 0.001 32.581** 2.493E-05
盐胁迫 0.245 0.048 0.468 10.273** 26.689** 0.000
接菌+盐胁迫 0.073 0.029 0.191 3.405* 5.180 0.001

Table 5

Correlation analysis of the effects of Hebeloma crustuliniforme on the growth indexes and physicochemical indexes of Hebeloma japonica under NaCl salt stress"

指标 苗高生长量 新梢生长量 分枝增长数 总鲜质量 总干质量 地下鲜质量 地上鲜质量 地下干质量 地上干质量 叶绿素a含量 叶绿素b含量 总叶绿素含量 相对含水量 脯氨酸含量 可溶性蛋白含量
苗高生长量 1
新梢生长量 0.153 1
分枝增长数 -0.048 -0.182 1
总鲜质量 0.104 0.277 0.305 1
总干质量 0.155 0.342* 0.325* 0.959** 1
地下鲜质量 -0.071 0.024 0.148 0.704** 0.489** 1
地上鲜质量 0.183 0.355* 0.316* 0.898** 0.977** 0.320* 1
地下干质量 -0.015 0.143 0.212 0.746** 0.635** 0.875** 0.453**
地上干质量 0.169 0.350** 0.323* 0.936** 0.995** 0.413** 0.993** 0.554** 1
叶绿素a含量 0.408* 0.168 0.101 -0.329 -0.280 -0.285 -0.290 -0.138 -0.286 1
叶绿素b含量 0.168 0.237 0.007 -0.187 -0.126 -0.276 -0.076 -0.218 -0.104 0.677** 1
总叶绿素含量 0.358 0.218 0.076 -0.306 -0.249 -0.304 -0.239 -0.176 -0.246 0.967** 0.841** 1
叶片相对含水量 0.096 -0.391 0.299 0.318 0.250 0.326 0.240 0.177 0.247 -0.336 -0.356 -0.369 1
脯氨酸含量 -0.157 0.297 -0.527** -0.381 -0.328 -0.352 -0.318 -0.227 -0.326 0.119 0.292 0.188 -0.409* 1
可溶性蛋白 -0.314 0.049 -0.245 0.048 0.060 0.072 0.019 0.154 0.042 -0.060 -0.214 -0.117 -0.228 -0.094 1
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