J4 ›› 2011, Vol. 46 ›› Issue (7): 17-20.

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AIsolation and structure identification of several holothurian glycosides compounds from the processing liquid waste of  sea cucumber Apostichopus japonicus

YUAN Wen-peng1, LIU Xin1, FAN Ting-jun2, ZHANG Yong-gang1, XIA Xue-kui1, LIU Chang-heng1*   

  1. 1. Biology Institute of Shandong Academy of Sciences, Key Laboratory For Applied Microbiology of Shandong Province,
    Jinan 250014, Shandong, China; 2. Department of Marine Biology, College of Marine Life Sciences,
    Ocean University of China, Qingdao 266003, Shandong, China
  • Received:2010-11-23 Online:2011-07-20 Published:2011-09-08

Abstract:

In order to explore higher applicative value of processing liquid waste of sea cucumber, holothurian glycosides compounds from the processing liquid waste of sea cucumber Apostichopus japonicus were investigated. The processing liquid waste was separated by macroporous resin chromatography, silica gel column chromatography and preparative high performance liquid chromatography to afford five compounds. On the basis of detailed spectroscopic analysis and comparison of the reported data in the literatures, they were elucidated as holotoxin D (1)、holotoxin B1(2)、holotoxin A (3)、holotoxin A1(4) and cladoloside B (5). All of the compounds were isolated from the Apostichopus japonicus processing liquid waste for the first time. This study laid a foundation for rational utilization and development of processing liquid waste of sea cucumber.

Key words: Apostichopus japonicus; processing liquid waste of sea cucumber; holothurian glycosides

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