Sequence analysis of mitochondrial 16S rRNA gene and molecular phylogeny of three species of abalones
- GUO Zhan-sheng, ZHANG Jun-wei, LI Liang, HOU Xu-guang, ZHANG Hai-tao, ZHANG Xue-jin
JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2017, 52(1):
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PCR method was used for amplification of mitochondrial 16S rRNA gene from H.discus hannai, H.iris and H.rubra, and all of amplicons were purified and sequenced. The results showed that 16S rRNA gene sequences with length of 544, 546 and 550 bp were obtained from H.discus hannai, H.iris and H.rubra, the content of A+T were 58.1%, 56.1% and 55.9% respectively, the mutual homology among them was 94.18%, the variation of the sequences focuses on the three regions: 9-34 bp, 248-295 bp and 326-375 bp. The cluster dendrograms of the three collected abalones and 22 abalones from GenBank data based on 16s rDNA were analysed, their genetic distance ranges from 0.00 to 0.14. The phylogenetic tree was constructed with NJ and ML methods to reveal the evolutionary relationships, the results showed that the phylogeny of Haliotide was easy to cluster in one clade when distributed in same district and had same chrosome numbers.