The growth of calli and regenerated plantlets of Gentiana Przewalskii Maxim. and the constituents analysis of its two effective components
- MA Jie-xiong,JIANG Li,QI Yu-yu,XIANG FEng-ning,XIA Guang-min
J4. 2006, 41(6):
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Vigorous grown embryonic calli and regenerated plants from Gentiana Przewalskii Maxim., a Chinese medicinal plant, are obtained by various media with different hormone combinations. The contents of swertiamarin and gentiopicroside of embryogenic calli, the regenerated plantlets and the wild plants in G. Przewalskii were determined by reverse phase high performance liquid chromatography (RPHPLC). The results show the induction and the growth rates of calli cultured on various media are different. In addition, the highest frequency (78.0%) of calli induced is observed on MS medium with 2?mg/L 2, 4D, 1?mg/L 6BA and 6?mg/L GA, while the fastest growth of embryonic calli is occurred on MS medium with 3mg/L 2,4D and 0.5?mg/L KT, which has increased 7.18 fold. Furthermore, the effective components in embryonic calli and the regenerated plants are different. The embryonic calli contains 0.265% of gentiopicroside, which is lower than that of the wild plant. The regenerated plants have 0.279% of swertiamarin and 1.579% of gentiopicroside, of which the former is higher and the latter is lower than that of the wild plant. Base on the speed of growth, the contents of the effective components accumulated from the calli and the regenerated plants are much higher than that from the wild plants.