《山东大学学报(理学版)》 ›› 2020, Vol. 55 ›› Issue (7): 103-110.doi: 10.6040/j.issn.1671-9352.0.2019.667
• • 上一篇
王胜男1(),栾兴社2,3,*(
),郭新娟3,肖敏4,戴美学1
Sheng-nan WANG1(),Xing-she LUAN2,3,*(
),Xin-juan GUO3,Min XIAO4,Mei-xue DAI1
摘要:
Klebsiella sp.LDX1-1多糖产生的最佳碳源为麦芽糖与低聚异麦芽糖的混合糖,其中低聚异麦芽糖质量分数为8%;最佳氮源为2g/L的NH4NO3。2g/L的雪松松针提取物、0.4%的曲拉通X-100显著促进多糖合成,产量分别提高12.46%和14.38%。用综合试验因素进行发酵,得到的最适发酵周期为24h,粗多糖产量达到23.46g/L,比发酵基础培养基条件下提高43.14%。由发酵液制取的初步纯化多糖具有高效絮凝活性,对高岭土悬液和黄河水的絮凝率分别是98.43%和96.31%。纯化样品的红外光谱定性分析表明:发酵产生的黏性物质成分为典型的生物多糖。
中图分类号:
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