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《山东大学学报(理学版)》 ›› 2021, Vol. 56 ›› Issue (7): 103-110.doi: 10.6040/j.issn.1671-9352.0.2021.002

• • 上一篇    

核磁共振指纹图谱用于阿胶的鉴别

邓书鸿1,4,郭传恩2*,姜红3**,聂磊1   

  1. 1. 山东大学药学院, 山东 济南 250012;2. 山东政法学院医院, 山东 济南 250014;3. 山东大学校医院, 山东 济南 250100;4. 齐鲁制药有限公司药物研究院, 山东 济南 250101
  • 发布日期:2021-07-19
  • 作者简介:邓书鸿(1987— ),男,硕士,研究方向为药物质量控制. E-mail: derek.sd@foxmail.com*并列第一作者**通信作者简介:姜红(1975— ),女,硕士,主治医师,研究方向为药品质量检验. E-mail:jianghong2004@sdu.edu.cn
  • 基金资助:
    国家科技重大专项“重大新药创制”项目(2018ZX09201010)

Study on the identification of Corii Colla Asini by nuclear magnetic resonance spectroscopy fingerprint

DENG Shu-hong1,4, GUO Chuan-en2*, JIANG Hong3**, NIE Lei1   

  1. 1. School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, China;
    2. Hospital of Shandong University of Political Science and Law, Jinan 250014, Shandong, China;
    3. Shandong University Hospital, Jinan 250100, Shandong, China;
    4. Pharmaceutical Research Institute, Qilu Pharmaceutical Co, Ltd, Jinan 250101, Shandong, China
  • Published:2021-07-19

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摘要： 建立阿胶核磁共振指纹图谱法用于阿胶的鉴别。基于阿胶水提液成分,采用核磁共振氢谱(1H-NMR)建立阿胶核磁共振指纹图谱方法,并进行方法学验证;对不同来源样品进行核磁共振氢谱测定,得到样品数据集。采用主成分分析(PCA)和片段偏最小二乘法(i-PLS)对样品进行模式识别研究,建立阿胶判别模型。结果显示,阿胶核磁共振指纹图谱方法的精密度、稳定性和重现性良好。PCA分析结果表明:不同来源样品有一定的类别差异,但阿胶真品与伪品难以分开。采用i-PLS法建立真伪阿胶判别模型,分类能力强,能够实现真伪阿胶的正确判别。阿胶核磁共振氢谱指纹图谱结合模式识别方法能够用于中药阿胶的真伪鉴别,为阿胶的质量控制及评价提供借鉴。

关键词: 阿胶, 核磁共振氢谱, 指纹图谱, 鉴别

Abstract: To establish the nuclear magnetic resonance(NMR)fingerprinting method and to identify the Corii Colla Asini(Ejiao)with NMR fingerprinting. The 1H-NMR based on the composition of the Corii Colla Asini water extract was used to establish its nuclear magnetic resonance fingerprint method, and the validation of method was also carried out. 1H nuclear magnetic resonance spectra were measured for samples from different sources to construct samples data sets. Principal component analysis(PCA)and interval partial least squares(i-PLS)were employed to conduct pattern recognition of samples and a discriminant model of Corii Colla Asini was accordingly built. The precision, stability and reproducibility of the Corii Colla Asini NMR fingerprinting method were good. The analysis results of PCA showed that there were certain categorical differences between samples from different sources, but it was difficult to distinguish the authentic and fake Corii Colla Asini samples. The i-PLS method was used to establish the discriminant model of Corii Colla Asini, which had a strong classification ability and could correctly discriminate the authentic Corii Colla Asini and its fake samples. The combination of the 1H NMR fingerprint and pattern recognition could be used to identify the authenticity of Corii Colla Asini, which would provide a significant reference for the quality control and evaluation of Corii Colla Asini.

Key words: Corii Colla Asini, 1H nuclear magnetic resonance spectrum, fingerprint, identification

中图分类号: 

  • O657.2
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