产广谱细菌素乳酸菌的筛选

产广谱细菌素乳酸菌的筛选

胡淑敏，孔健，季明杰*

山东大学微生物技术国家重点实验室，山东济南 250100

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-10-24 发布日期:2006-10-24
通讯作者: 季明杰

Screening of broad-spectrum bacteriocin-producing lactic acid bacteria

HU Shu-min, KONG Jian, JI Ming-jie*

State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong, China

Received:1900-01-01 Revised:1900-01-01 Online:2006-10-24 Published:2006-10-24
Contact: JI Ming-jie

摘要/Abstract

摘要：

利用牛津杯双层平板法从70株乳酸菌中筛选得到2株具有较高抑菌活性的乳酸菌LAB30、LAB211。研究这2株乳酸菌的抑菌物质发现：排除酸性产物乳酸、乙酸以及过氧化氢的干扰，LAB211和LAB30仍具有较高的抑菌活性；对其发酵液进行蛋白酶处理，LAB30的发酵液经胰蛋白酶和蛋白酶K处理后抑菌活性均有降低；而LAB211的发酵液经蛋白酶K处理后有所降低，胰蛋白酶处理没有变化，因此可以判定这两株乳酸菌产生的抑菌物质为细菌素。利用特异性引物PCR分析可知，LAB211和LAB30产生的细菌素并非nisin。抑菌谱实验表明，LAB30和LAB211的发酵液不仅抑制革兰氏阳性菌，而且对部分革兰氏阴性菌也有抑制作用。因此LAB211和LAB30产生的是一类广谱细菌素。

关键词: 乳酸菌, 抑菌试验 , 细菌素

Abstract:

LAB211 and LAB30 strains, which showed obvious micro-biostatic effects, were selected from 70 Lactic acid bacteria isolated from fresh milk using the agar diffusion method. The supernatants of LAB211 and LAB30 cultures eliminated the effects of organic acid and hydrogen peroxide, and still exhibited inhibitor activity against the gram-positive bacteria. However, the inhibitory activity of LAB30 stain decreased after treatment with protease K and trypsin, while the inhibitory activity of LAB211 strain decreased by treatment with protease K, which showed that this inhibitory material had the feature of protein. Thus, the inhibitory material produced by LAB211 and LAB30 could be classed as bacteriocin. PCR analysis showed that the inhibitory materials were not nisin. The inhibitory spectrum proved that the supernatants of LAB211 and LAB30 could inhibit not only gram-positive bacteria but also partial gram-negative bacteria. Therefore, these two wide inhibitory spectrum bacteriocin-producing Lactic acid bacteria strains were isolated.

Key words: antibacterial experiment

, bacteriocin,

Lactic acid bacteria

中图分类号:

Q93-3

胡淑敏,孔健,季明杰* . 产广谱细菌素乳酸菌的筛选[J]. J4, 2008, 43(7): 61-64 .

HU Shu-min,KONG Jian,JI Ming-jie* .

Screening of broad-spectrum bacteriocin-producing lactic acid bacteria

[J]. J4, 2008, 43(7): 61-64 .

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