超嗜热古菌Sulfolobus tokodaii尿嘧啶营养缺陷型筛选条件的最适化及初步筛选

超嗜热古菌Sulfolobus tokodaii尿嘧啶营养缺陷型筛选条件的最适化及初步筛选

黄奇洪，申玉龙，倪金凤*

山东大学微生物技术国家重点实验室，山东济南 250100

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-10-24 发布日期:2006-10-24
通讯作者: 倪金凤

Optimization of selective conditions for the selection of uracil auxotrophs of thermophilic archaea Sulfolobus tokodaii

HUANG Qi-hong, SHEN Yu-long, NI Jin-feng*

State Key Lab of Microbial Technology, Shandong University, Jinan 250100, Shandong, China

Received:1900-01-01 Revised:1900-01-01 Online:2006-10-24 Published:2006-10-24
Contact: NI Jin-feng

摘要/Abstract

摘要： 超嗜热古菌Sulfolobus tokodaii隶属于古菌中的泉古菌（Crenarchaea），硫化叶菌属（Sulfolobus）。野生型S.tokodaii尿嘧啶相关基因表达的乳清核苷酸转移酶和乳清苷单磷酸脱羧酶可以将5-氟乳清酸(5-FOA)转化成有毒物质5-氟尿嘧啶核苷酸，导致野生型S.tokodaii无法正常生长。根据此原理，通过对筛选条件如5-FOA的质量浓度、紫外诱变时间等的最适化，运用微生物的自发突变或对其进行紫外照射等诱变方法，初步筛选出S.tokodaii的尿嘧啶营养缺陷型菌株。

关键词: Sulfolobus tokodaii, 紫外诱变, 5-氟乳清酸, 尿嘧啶营养缺陷型, 菌种筛选

Abstract: Thermophilic archaea Sulfolobus tokodaii was recognized as a species of the genus Sulfolobus, which belongs to Crenarchaea of Archaea. Orotate phosphoribosyl transferase and orotidine monophosphate decarboxylase, expressed by a uracil-related gene in the wild type of Sulfolobus tokodaii, could transform 5-fluoroorotic acid (5-FOA) to the toxic substance 5-fluorouridine monophosphate. Therefore, the wild-type strain could not grow normally. The selective condition was optimized based on this theory, such as the effective concentration of 5-FOA and the time of UV irradiation, and several uracil auxotrophs mutants of S.tokodaii were primarily obtained by spontaneous change or UV mutagenesis.

Key words: UV mutagenesis, 5-FOA, uracil auxotrophs, strains selection, Sulfolobus tokodaii

中图分类号:

Q935

黄奇洪,申玉龙,倪金凤* . 超嗜热古菌Sulfolobus tokodaii尿嘧啶营养缺陷型筛选条件的最适化及初步筛选[J]. J4, 2008, 43(9): 6-10 .

HUANG Qi-hong,SHEN Yu-long,NI Jin-feng* . Optimization of selective conditions for the selection of uracil auxotrophs of thermophilic archaea Sulfolobus tokodaii[J]. J4, 2008, 43(9): 6-10 .

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