济宁青山羊大小猾子皮的差异表达基因研究

济宁青山羊大小猾子皮的差异表达基因研究

张兆远¹，王树迎¹，王慧¹,赵晶²

1．山东农业大学动物科技学院，山东泰安 271018；
2. 山东大学生命科学院，山东济南 250100

收稿日期:2008-07-02 修回日期:1900-01-01 出版日期:2009-04-24 发布日期:2009-11-26
通讯作者: 王慧

Studies on differential expressed genes between older and young goat skins of the Jining grey goat

ZHANG Zhaoyuan ¹, WANG Shuying ¹, WANG Hui ¹, ZHAO Jing ²

1. College of Animal Science ＆ Technology, Shandong Agricultural University, Taian 271018, Shandong, China;
2. College of Biology, Shandong University, Jinan 250100, Shandong, China

Received:2008-07-02 Revised:1900-01-01 Online:2009-04-24 Published:2009-11-26

摘要/Abstract

摘要：

以差异显示PCR（DDRTPCR）技术研究了小猾皮（出生3?d内）和大猾皮（出生30～60?d）的差异表达基因，并以反Northern点杂交技术快速剔除假

阳性条带。从40对随机引物中筛选出8对引物组合，其在2种猾子皮中共扩增出482条cDNA片段，其中差异表达条带为52条，占总条带数的10.79％。在所发现的差异条带中有41条为表达“强与弱”的差异

带，占总条带数的8.51％；另有11条为表达“有或无”的差异条带，占总条带数的2.28％。说明与小猾皮相比，大猾皮皮肤组织中有8.51%的基因增强或减弱表达，有2.28％的基因启动或关闭表达。这些

差异表达的基因是大猾皮和小猾皮花纹品质差异的遗传基础。

关键词: 基因, 差异显示PCR, 济宁青山羊, 猾子皮

Abstract:

DDRTPCR and reverse Northern dot hybridization methods were used to detect the different expressed genes between kid lambs（within 3 days old）and the older lambs skins (from

30 to 60 days old) of the Jining Grey Goat. The false bands were quickly rejected by reverse Northern dot hybridization. The results indicated that 482 cDNA fragments were

isolated using 8 kinds of primer combinations selected from 40 pairs of random primer with DDRTPCR. 52 differential expressed bands (10.79%) were discovered, of which 41 bands

(8.51%) were differentely expressed in amount between kid lambs' skin and those of the elder lambs' skin, and there were 11 newly expressed or closed genes (2.28%) of those

found. This means that compared to kid lambs, older lambs have 8.51% gene expressed difference in amount, and 2.28% new genes appeared or closed in the skins. The differential

expressed genes would be the genetic basis of figure quality between kid lambs and older lambs skins.

Key words: Jining grey goats, kid lamb's skin, genes, DDRTPCR

中图分类号:

张兆远1 ，王树迎1 ，王慧1 ,赵晶2 .

济宁青山羊大小猾子皮的差异表达基因研究

[J]. J4, 2009, 44(3): 7-10 .

ZHANG Zhaoyuan ¹, WANG Shuying ¹, WANG Hui ¹, ZHAO Jing ². Studies on differential expressed genes between older and young goat skins of the Jining grey goat[J]. J4, 2009, 44(3): 7-10 .

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