关键词: 农杆菌；番茄；几丁质酶；葡聚糖酶；抗病性；遗传转化

Abstract:

A plant expression vector pBLGC containing two disease-resistant genes, the Chitinase gene and the Glutanase gene, was introduced into cotyledon explants of tomato via Agrobacterium-mediated genetic transformation. After co-culture, kanamycin selection and regeneration, 23 kanamycin-resistant plants were obtained. Among these, 7 plants were demonstrated to be transgenics by PCR and PCR-Southern Blot analysis. Through pathogenic fungus inoculation, it was shown that 3 transgenic plants had strong disease resistance. These results will lay an important basis for the further study of tomato disease resistance and for tomato genetic improvement.
 

Key words: Agrobacterium tumefaciens; tomato; chitinase; glucanase; disease resistance; genetic transformation